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2.A.66.3.1
The OLF (Rft1 protein) of Saccharomyces cerevisiae.  May play a role in phospholipid flipping from the inner leaflet of the plasma membrane to the outer leaflet (Chauhan et al. 2016).

Accession Number:P38206
Protein Name:Rft1 aka YBL020W aka YBL0442
Length:574
Molecular Weight:66211.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Multi-pass membrane protein2
Substrate phospholipids

Cross database links:

Genevestigator: P38206
eggNOG: fuNOG04763
HEGENOM: HBG397989
DIP: DIP-3885N
RefSeq: NP_009533.1   
Entrez Gene ID: 852261   
Pfam: PF04506   
KEGG: sce:YBL020W   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0016021 C:integral to membrane
GO:0005319 F:lipid transporter activity
GO:0005515 F:protein binding
GO:0008643 P:carbohydrate transport
GO:0034203 P:glycolipid translocation
GO:0006487 P:protein amino acid N-linked glycosylation

References (5)

[1] “Suppression of the yeast mutation rft1-1 by human p53.”  Koerte A.et.al.   7673248
[2] “Complete DNA sequence of yeast chromosome II.”  Feldmann H.et.al.   7813418
[3] “The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts.”  Hahn S.et.al.   2832732
[4] “Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein.”  Helenius J.et.al.   11807558
[5] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAKKNSQLPS TSEQILERST TGATFLMMGQ LFTKLVTFIL NNLLIRFLSP RIFGITAFLE 
61:	FIQGTVLFFS RDAIRLSTLR ISDSGNGIID DDDEEEYQET HYKSKVLQTA VNFAYIPFWI 
121:	GFPLSIGLIA WQYRNINAYF ITLPFFRWSI FLIWLSIIVE LLSEPFFIVN QFMLNYAARS 
181:	RFESIAVTTG CIVNFIVVYA VQQSRYPMGV VTSDIDKEGI AILAFALGKL AHSITLLACY 
241:	YWDYLKNFKP KKLFSTRLTK IKTRENNELK KGYPKSTSYF FQNDILQHFK KVYFQLCFKH 
301:	LLTEGDKLII NSLCTVEEQG IYALLSNYGS LLTRLLFAPI EESLRLFLAR LLSSHNPKNL 
361:	KLSIEVLVNL TRFYIYLSLM IIVFGPANSS FLLQFLIGSK WSTTSVLDTI RVYCFYIPFL 
421:	SLNGIFEAFF QSVATGDQIL KHSYFMMAFS GIFLLNSWLL IEKLKLSIEG LILSNIINMV 
481:	LRILYCGVFL NKFHRELFTD SSFFFNFKDF KTVIIAGSTI CLLDWWFIGY VKNLQQFVVN 
541:	VLFAMGLLAL ILVKERQTIQ SFINKRAVSN SKDV