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2.A.7.13.1
Golgi GDP-mannose:GMP antiporter, (vanadate resistance protein), VRG4 or VIG4 (Abe et al. 1999). It is the target of a natural cyclic peptide of unknown function (SDZ 90-215) (Snyder et al. 2019).

Accession Number:P40107
Protein Name:GOG5 aka VRG4 aka VAN2 aka VIG4 aka MCD3 aka YGL225W
Length:337
Molecular Weight:37019.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate GDP-mannose

Cross database links:

Genevestigator: P40107
HEGENOM: HBG328976
DIP: DIP-5108N
RefSeq: NP_011290.1   
Entrez Gene ID: 852647   
Pfam: PF08449   
KEGG: sce:YGL225W   

Gene Ontology

GO:0030659 C:cytoplasmic vesicle membrane
GO:0005789 C:endoplasmic reticulum membrane
GO:0000139 C:Golgi membrane
GO:0016021 C:integral to membrane
GO:0005739 C:mitochondrion
GO:0005338 F:nucleotide-sugar transmembrane transporter ...
GO:0005515 F:protein binding
GO:0030259 P:lipid glycosylation
GO:0015780 P:nucleotide-sugar transport
GO:0006487 P:protein amino acid N-linked glycosylation
GO:0055085 P:transmembrane transport

References (18)

[1] “The yeast VRG4 gene is required for normal Golgi functions and defines a new family of related genes.”  Poster J.B.et.al.   8632002
[2] “Sodium orthovanadate-resistant mutants of Saccharomyces cerevisiae show defects in Golgi-mediated protein glycosylation, sporulation and detergent resistance.”  Kanik-Ennulat C.et.al.   7672592
[3] “Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII.”  Rieger M.et.al.   9290212
[4] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[5] “Vanadate-resistant mutants of Saccharomyces cerevisiae show alterations in protein phosphorylation and growth control.”  Kanik-Ennulat C.et.al.   2137555
[6] “Vanadate-resistant yeast mutants are defective in protein glycosylation.”  Ballou L.et.al.   2014241
[7] “Yeast glycosylation mutants are sensitive to aminoglycosides.”  Dean N.et.al.   7877969
[8] “Identification of genes controlling growth polarity in the budding yeast Saccharomyces cerevisiae: a possible role of N-glycosylation and involvement of the exocyst complex.”  Mondesert G.et.al.   9335583
[9] “Isolation of new nonconditional Saccharomyces cerevisiae mutants defective in asparagine-linked glycosylation.”  Manas P.et.al.   9184829
[10] “The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae.”  Dean N.et.al.   9395539
[11] “Molecular characterization of Vig4/Vrg4 GDP-mannose transporter of the yeast Saccharomyces cerevisiae.”  Abe M.et.al.   10570930
[12] “Identification of a conserved motif in the yeast golgi GDP-mannose transporter required for binding to nucleotide sugar.”  Gao X.-D.et.al.   11067855
[13] “Progression of the stacked Golgi compartments in the yeast Saccharomyces cerevisiae by overproduction of GDP-mannose transporter.”  Hashimoto H.et.al.   12478588
[14] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[15] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[16] “Localization of GDP-mannose transporter in the Golgi requires retrieval to the endoplasmic reticulum depending on its cytoplasmic tail and coatomer.”  Abe M.et.al.   15494368
[17] “Golgi maturation visualized in living yeast.”  Losev E.et.al.   16699524
[18] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSELKTGHAG HNPWASVANS GPISILSYCG SSILMTVTNK FVVNLKDFNM NFVMLFVQSL 
61:	VCTITLIILR ILGYAKFRSL NKTDAKNWFP ISFLLVLMIY TSSKALQYLA VPIYTIFKNL 
121:	TIILIAYGEV LFFGGSVTSM ELSSFLLMVL SSVVATWGDQ QAVAAKAASL AEGAAGAVAS 
181:	FNPGYFWMFT NCITSALFVL IMRKRIKLTN FKDFDTMFYN NVLALPILLL FSFCVEDWSS 
241:	VNLTNNFSND SLTAMIISGV ASVGISYCSG WCVRVTSSTT YSMVGALNKL PIALSGLIFF 
301:	DAPRNFLSIL SIFIGFLSGI IYAVAKQKKQ QAQPLRK