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2.A.76.1.5
The leucine exporter (LeuE; YeaS) (binds and probably transports L-methionine, L-histidine, L-α-amino-n-butyrate, norleucine, azaleucine, S(2-aminoethyl)-L-cysteine, 3,4-dehydro-DL-proline, L-alanine, and both D- and L-valine) (Kutukova et al., 2005; Hori et al., 2011).  Expression is under the control of the leucine-responsive protein, Lrp, and amino acids in the medium (Kutukova et al. 2005).

Accession Number:P76249
Protein Name:Hypothetical protein yeaS aka YeaS
Length:212
Molecular Weight:23201.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Leucine

Cross database links:

Genevestigator: P76249
EchoBASE: EB3278
EcoGene: EG13505
eggNOG: COG1280
HEGENOM: HBG759042
RefSeq: AP_002417.1    NP_416312.1   
Entrez Gene ID: 946157   
Pfam: PF01810   
BioCyc: EcoCyc:G6984-MONOMER    ECOL168927:B1798-MONOMER   
KEGG: ecj:JW1787    eco:b1798   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015190 F:L-leucine transmembrane transporter activity
GO:0015820 P:leucine transport

References (4)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The yeaS (leuE) gene of Escherichia coli encodes an exporter of leucine, and the Lrp protein regulates its expression.”  Kutukova E.A.et.al.   16098526

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFAEYGVLNY WTYLVGAIFI VLVPGPNTLF VLKNSVSSGM KGGYLAACGV FIGDAVLMFL 
61:	AWAGVATLIK TTPILFNIVR YLGAFYLLYL GSKILYATLK GKNSEAKSDE PQYGAIFKRA 
121:	LILSLTNPKA ILFYVSFFVQ FIDVNAPHTG ISFFILAATL ELVSFCYLSF LIISGAFVTQ 
181:	YIRTKKKLAK VGNSLIGLMF VGFAARLATL QS