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3.A.1.106.20
MsbA of 582 aas and 6 TMSs in an M-C arrangement.  The X-ray structure at 2.8 Å resolution in an inward-facing conformation after cocrystallization with lipid A and using a stabilizing facial amphiphile has been reported (Padayatti et al. 2019). The structure displays a large amplitude opening in the transmembrane portal, which is likely to be required for lipid A to pass from its site of synthesis into the protein-enclosed transport pathway. Putative lipid A density is observed further inside the transmembrane cavity, consistent with a trap and flip model. Additional electron density attributed to lipid A is observed near an outer surface cleft at the periplasmic ends of the transmembrane helices (Padayatti et al. 2019). This protein is 96% identical to the E. coli ortholog, TC# 3.A.1.106.1.

Accession Number:P63359
Protein Name:Lipid A export ATP-binding/permease protein MsbA
Length:582
Molecular Weight:64340.00
Species:Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [99287]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate

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FASTA formatted sequence
1:	MHNDKDLSTW QTFRRLWPTI APFKAGLIVA GIALILNAAS DTFMLSLLKP LLDDGFGKTD 
61:	RSVLLWMPLV VIGLMILRGI TSYISSYCIS WVSGKVVMTM RRRLFGHMMG MPVAFFDKQS 
121:	TGTLLSRITY DSEQVASSSS GALITVVREG ASIIGLFIMM FYYSWQLSII LVVLAPIVSI 
181:	AIRVVSKRFR SISKNMQNTM GQVTTSAEQM LKGHKEVLIF GGQEVETKRF DKVSNKMRLQ 
241:	GMKMVSASSI SDPIIQLIAS LALAFVLYAA SFPSVMDSLT AGTITVVFSS MIALMRPLKS 
301:	LTNVNAQFQR GMAACQTLFA ILDSEQEKDE GKRVIDRATG DLEFRNVTFT YPGREVPALR 
361:	NINLKIPAGK TVALVGRSGS GKSTIASLIT RFYDIDEGHI LMDGHDLREY TLASLRNQVA 
421:	LVSQNVHLFN DTVANNIAYA RTEEYSREQI EEAARMAYAM DFINKMDNGL DTIIGENGVL 
481:	LSGGQRQRIA IARALLRDSP ILILDEATSA LDTESERAIQ AALDELQKNR TSLVIAHRLS 
541:	TIEQADEIVV VEDGIIVERG THSELLAQHG VYAQLHKMQF GQ