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3.A.1.112.9
ABC bacteriocin exporter with two peptidase domains, Pcat1. The 3-D structure is known (4S0F). The pathway for peptide export consists of an large α-helical barrel for small folded peptides.  ATP binding alternates access to the transmembrane pathway and reglates protease activity (Lin et al. 2015). Subunit asymmetry of the M3-M4 loops contribute to optimizing AChR activation through allosteric links to the channel and the agonist binding site (Shen et al. 2005). Structures were more recently determined in the absence and presence of ATP, revealing how ATP binding regulates the protease activity and access to the translocation pathway. Two substrate CtAs, a 90-residue polypeptide, are bound via their N-terminal leader peptides, but only one is positioned for cleavage and translocation. The structures were determined in the absence and presence of ATP, revealing how ATP binding regulates the protease activity and access to the translocation pathway. It seems that substrate cleavage, ATP hydrolysis, and substrate translocation are coordinated in a transport cycle (Kieuvongngam et al. 2020).

Accession Number:A3DCU1
Protein Name:ABC-type bacteriocin transporter
Length:727
Molecular Weight:80816.00
Species:Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) [203119]
Number of TMSs:6
Substrate Bacteriocins

Cross database links:

Structure:
4RY2   4S0F   6V9Z     

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FASTA formatted sequence
1:	MLRRLFKKKY VCVRQYDLTD CGAACLSSIA QYYGLKMSLA KIREMTGTDT QGTNAYGLIH 
61:	AAKQLGFSAK GVKASKEDLL KDFRLPAIAN VIVDNRLAHF VVIYSIKNRI ITVADPGKGI 
121:	VRYSMDDFCS IWTGGLVLLE PGEAFQKGDY TQNMMVKFAG FLKPLKKTVL CIFLASLLYT 
181:	ALGIAGSFYI KFLFDDLIKF EKLNDLHIIS AGFAVIFLLQ IFLNYYRSIL VTKLGMSIDK 
241:	SIMMEYYSHV LKLPMNFFNS RKVGEIISRF MDASKIRQAI SGATLTIMID TIMAVIGGIL 
301:	LYIQNSSLFF ISFIIILLYG IIVTVFNKPI QNANRQIMED NAKLTSALVE SVKGIETIKS 
361:	FGAEEQTEKS TRDKIETVMK SSFKEGMLYI NLSSLTGIVA GLGGIVILWA GAYNVIKGNM 
421:	SGGQLLAFNA LLAYFLTPVK NLIDLQPLIQ TAVVASNRLG EILELATEKE LREDSDDFVI 
481:	SLKGDIEFRN VDFRYGLRKP VLKNINLTIP KGKTVAIVGE SGSGKTTLAK LLMNFYSPEK 
541:	GDILINGHSI KNISLELIRK KIAFVSQDVF IFSGTVKENL CLGNENVDMD EIIKAAKMAN 
601:	AHDFIEKLPL KYDTFLNESG ANLSEGQKQR LAIARALLKK PDILILDEAT SNLDSITENH 
661:	IKDAIYGLED DVTVIIIAHR LSTIVNCDKI YLLKDGEIVE SGSHTELIAL KGCYFKMWKQ 
721:	TENTLAS