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3.A.1.120.8
 ABC protein of 558 aas and 0 TMSs, Rv2477c.  It is a translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA (fMet) in the P site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E site where it modulates the state of the translating ribosome during subunit translocation. It is an ABC-F subfamily protein, members of which are implicated in diverse cellular processes such as translation, antibiotic resistance, cell growth and nutrient sensing. Daniel et al. 2018 showed that Rv2477c displays strong ATPase activity (Vmax = 45 nmol/mg/min; Km = 90 muM) that is sensitive to orthovanadate. The ATPase activity was maximal in the presence of Mn2+ at pH 5.2. The protein hydrolyzed GTP, TTP and CTPas well as ATP but at lower rates. Glutamate to glutamine substitutions of amino acid residues 185 and 468 in the two Walker B motifs severely inhibited its ATPase activity. The antibiotics, tetracycline and erythromycin, which target protein translation, were able to inhibit the ATPase activity. Daniel et al. 2018 postulated that Rv2477c is involved in mycobacterial protein translation and in resistance to tetracyclines and macrolides.

Accession Number:P9WQK3
Protein Name:Energy-dependent translational throttle protein EttA
Length:558
Molecular Weight:61893.00
Species:Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) [83332]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate

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FASTA formatted sequence
1:	MAEFIYTMKK VRKAHGDKVI LDDVTLSFYP GAKIGVVGPN GAGKSSVLRI MAGLDKPNNG 
61:	DAFLATGATV GILQQEPPLN EDKTVRGNVE EGMGDIKIKL DRFNEVAELM ATDYTDELME 
121:	EMGRLQEELD HADAWDLDAQ LEQAMDALRC PPADEPVTNL SGGERRRVAL CKLLLSKPDL 
181:	LLLDEPTNHL DAESVQWLEQ HLASYPGAIL AVTHDRYFLD NVAEWILELD RGRAYPYEGN 
241:	YSTYLEKKAE RLAVQGRKDA KLQKRLTEEL AWVRSGAKAR QAKSKARLQR YEEMAAEAEK 
301:	TRKLDFEEIQ IPVGPRLGNV VVEVDHLDKG YDGRALIKDL SFSLPRNGIV GVIGPNGVGK 
361:	TTLFKTIVGL ETPDSGSVKV GETVKLSYVD QARAGIDPRK TVWEVVSDGL DYIQVGQTEV 
421:	PSRAYVSAFG FKGPDQQKPA GVLSGGERNR LNLALTLKQG GNLILLDEPT NDLDVETLGS 
481:	LENALLNFPG CAVVISHDRW FLDRTCTHIL AWEGDDDNEA KWFWFEGNFG AYEENKVERL 
541:	GVDAARPHRV THRKLTRG