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3.A.1.208.11
Vacuolar metal resistance and drug detoxification protein, yeast cadmium factor (YCF1); transports cadmium-glutathione conjugates, glutathione S-conjugated leukotriene C4, organic glutathione S-conjugates, selenodigluthatione, unconjugated bilirubin, reduced glutathione, and diazaborine (Lazard et al., 2011). Mediates arsenite expulsion, possibly as a glutathione conjugate. Activity is dependent on Tus1p, a guanine nucleotide exchange factor (GEF) for the small GTPase Rho1p and a Rho1p-dependent-positive regulator of Ycf1p (Paumi et al. 2007).  

Accession Number:P39109
Protein Name:YCFI aka YCF1 aka YDR135C aka YD9302.11C
Length:1515
Molecular Weight:171121.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Metal ions, drugs, Cd2+-glutathione conjugates, Glutathione S-conjugated leukotriene C4, Glutathione S-conjugates, Selenodiglutathione, Unconjugated bilirubin, Reduced glutathione, Diaizaborine, arsenite

Cross database links:

Genevestigator: P39109
eggNOG: fuNOG05354
HEGENOM: HBG758042
DIP: DIP-2612N
RefSeq: NP_010419.1   
Entrez Gene ID: 851713   
Pfam: PF00664    PF00005   
KEGG: sce:YDR135C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0005524 F:ATP binding
GO:0015127 F:bilirubin transmembrane transporter activity
GO:0015431 F:glutathione S-conjugate-exporting ATPase ac...
GO:0005515 F:protein binding
GO:0015723 P:bilirubin transport
GO:0046686 P:response to cadmium ion
GO:0042493 P:response to drug
GO:0055085 P:transmembrane transport

References (12)

[1] “A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein.”  Szczypka M.S.et.al.   7521334
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[3] “The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump.”  Li Z.S.et.al.   8626454
[4] “The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae.”  Petrovic S.et.al.   10790694
[5] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[6] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[7] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[8] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[9] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[10] “Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.”  Chi A.et.al.   17287358
[11] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[12] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAGNLVSWAC KLCRSPEGFG PISFYGDFTQ CFIDGVILNL SAIFMITFGI RDLVNLCKKK 
61:	HSGIKYRRNW IIVSRMALVL LEIAFVSLAS LNISKEEAEN FTIVSQYAST MLSLFVALAL 
121:	HWIEYDRSVV ANTVLLFYWL FETFGNFAKL INILIRHTYE GIWYSGQTGF ILTLFQVITC 
181:	ASILLLEALP KKPLMPHQHI HQTLTRRKPN PYDSANIFSR ITFSWMSGLM KTGYEKYLVE 
241:	ADLYKLPRNF SSEELSQKLE KNWENELKQK SNPSLSWAIC RTFGSKMLLA AFFKAIHDVL 
301:	AFTQPQLLRI LIKFVTDYNS ERQDDHSSLQ GFENNHPQKL PIVRGFLIAF AMFLVGFTQT 
361:	SVLHQYFLNV FNTGMYIKSA LTALIYQKSL VLSNEASGLS STGDIVNLMS VDVQKLQDLT 
421:	QWLNLIWSGP FQIIICLYSL YKLLGNSMWV GVIILVIMMP LNSFLMRIQK KLQKSQMKYK 
481:	DERTRVISEI LNNIKSLKLY AWEKPYREKL EEVRNNKELK NLTKLGCYMA VTSFQFNIVP 
541:	FLVSCCTFAV FVYTEDRALT TDLVFPALTL FNLLSFPLMI IPMVLNSFIE ASVSIGRLFT 
601:	FFTNEELQPD SVQRLPKVKN IGDVAINIGD DATFLWQRKP EYKVALKNIN FQAKKGNLTC 
661:	IVGKVGSGKT ALLSCMLGDL FRVKGFATVH GSVAYVSQVP WIMNGTVKEN ILFGHRYDAE 
721:	FYEKTIKACA LTIDLAILMD GDKTLVGEKG ISLSGGQKAR LSLARAVYAR ADTYLLDDPL 
781:	AAVDEHVARH LIEHVLGPNG LLHTKTKVLA TNKVSALSIA DSIALLDNGE ITQQGTYDEI 
841:	TKDADSPLWK LLNNYGKKNN GKSNEFGDSS ESSVRESSIP VEGELEQLQK LNDLDFGNSD 
901:	AISLRRASDA TLGSIDFGDD ENIAKREHRE QGKVKWNIYL EYAKACNPKS VCVFILFIVI 
961:	SMFLSVMGNV WLKHWSEVNS RYGSNPNAAR YLAIYFALGI GSALATLIQT IVLWVFCTIH 
1021:	ASKYLHNLMT NSVLRAPMTF FETTPIGRIL NRFSNDIYKV DALLGRTFSQ FFVNAVKVTF 
1081:	TITVICATTW QFIFIIIPLS VFYIYYQQYY LRTSRELRRL DSITRSPIYS HFQETLGGLA 
1141:	TVRGYSQQKR FSHINQCRID NNMSAFYPSI NANRWLAYRL ELIGSIIILG AATLSVFRLK 
1201:	QGTLTAGMVG LSLSYALQIT QTLNWIVRMT VEVETNIVSV ERIKEYADLK SEAPLIVEGH 
1261:	RPPKEWPSQG DIKFNNYSTR YRPELDLVLK HINIHIKPNE KVGIVGRTGA GKSSLTLALF 
1321:	RMIEASEGNI VIDNIAINEI GLYDLRHKLS IIPQDSQVFE GTVRENIDPI NQYTDEAIWR 
1381:	ALELSHLKEH VLSMSNDGLD AQLTEGGGNL SVGQRQLLCL ARAMLVPSKI LVLDEATAAV 
1441:	DVETDKVVQE TIRTAFKDRT ILTIAHRLNT IMDSDRIIVL DNGKVAEFDS PGQLLSDNKS 
1501:	LFYSLCMEAG LVNEN