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3.A.1.208.17
The vacuolar glutathione-conjugate and chlorophyll catabolite transporter, MRP3 (Tommasini et al., 1998).  This protein appears to have an M-M-C-M-C domain order, possibly a characteristic of this ABC subfamily.  It exports reduced and oxidized glutathione (GSH) as well as GSH conjugates of cadmium, dinitrophenol, metolachlor, herbicies and anthocyanins (Lu et al. 1997).

Accession Number:Q9LK64
Protein Name:Multidrug resistance-associated protein 3 aka EST2 aka MRP3
Length:1514
Molecular Weight:168971.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:17
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Glutathione conjugates, Chlorophyll catabolites

Cross database links:

Genevestigator: Q9LK64
eggNOG: KOG0054
HEGENOM: HBG758042
RefSeq: NP_187915.1    NP_850575.1   
Entrez Gene ID: 820496   
Pfam: PF00664    PF00005   
KEGG: ath:AT3G13080   

Gene Ontology

GO:0048046 C:apoplast
GO:0016021 C:integral to membrane
GO:0000325 C:plant-type vacuole
GO:0005886 C:plasma membrane
GO:0005774 C:vacuolar membrane
GO:0005524 F:ATP binding
GO:0010290 F:chlorophyll catabolite transmembrane transp...
GO:0015431 F:glutathione S-conjugate-exporting ATPase ac...
GO:0008559 F:xenobiotic-transporting ATPase activity
GO:0055085 P:transmembrane transport

References (8)

[1] “Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics.”  Tommasini R.et.al.   9271206
[2] “Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones.”  Kaneko T.et.al.   10907853
[3] “Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana.”  Sanchez-Fernandez R.et.al.   9671034
[4] “An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity.”  Tommasini R.et.al.   9681016
[5] “The Arabidopsis thaliana ABC protein superfamily, a complete inventory.”  Sanchez-Fernandez R.et.al.   11346655
[6] “Multifunctionality of plant ABC transporters -- more than just detoxifiers.”  Martinoia E.et.al.   11855639
[7] “Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana.”  Kolukisaoglu U.H.et.al.   12430019
[8] “Plant ABC proteins - a unified nomenclature and updated inventory.”  Verrier P.J.et.al.   18299247

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDFLGSTTGS GTLAMLFSFS ESILPLDSRS FLLKPLFLRW LSGFLHSVLL LVLFFSWVRK 
61:	KIRGDSGVTE SLKDRRDFGF KSALFCSLAL SLLNLVLMSL SGFYWYESGW LDNEQLVSSL 
121:	GFLLGMVSWG VLSICLHRCR DCEHKKAPFL LRLWLVFYLV VSCYSLVVDF VMYERRETVP 
181:	VHLLVFDIVA FIAAVFLGYV AVLKKDRSNS NGVLEEPLLN GGDSRVGGDD SVELNKTNGS 
241:	GEATPYSRAG ILSLLTFSWM SPLIDIGNKK TLDLEDVPQL HDTDSVVGLA PKFRSMLESP 
301:	DGGERSGVTT FKLIKALYFT AQWEILVTAF FAFIYTVASY VGPALIDTFV QYLNGRRQYN 
361:	HEGYVLVITF FAAKIVECLS QRHWFFRLQK VGIRMRSALV AMIYEKGLTL SCQSKQGRTS 
421:	GEIINFMTVD AERIGNFSWY MHDPWMVLLQ VGLALWILYR NLGLASIAAL VATIIVMLIN 
481:	FPFGRMQERF QEKLMEAKDS RMKSTSEILR NMRILKLQGW EMKFLSKIFD LRKSEEGWLK 
541:	KYVYNSAVIS FVFWGAPTLV SVSTFGACIL LGIPLESGKI LSALATFRIL QEPIYNLPDT 
601:	ISMIVQTKVS LDRLASYLCL DNLQPDIVER LPKGSSDVAV EVINSTLSWD VSSSNPTLKD 
661:	INFKVFPGMK VAVCGTVGSG KSSLLSSLLG EVPKVSGSLK VCGTKAYVAQ SPWIQSGKIE 
721:	DNILFGKPME RERYDKVLEA CSLSKDLEIL SFGDQTVIGE RGINLSGGQK QRIQIARALY 
781:	QDADIYLFDD PFSAVDAHTG SHLFKEVLLG LLCSKSVIYV THQVEFLPAA DLILVMKDGR 
841:	ISQAGKYNDI LNSGTDFMEL IGAHQEALAV VDSVDANSVS EKSALGQENV IVKDAIAVDE 
901:	KLESQDLKND KLESVEPQRQ IIQEEEREKG SVALDVYWKY ITLAYGGALV PFILLGQVLF 
961:	QLLQIGSNYW MAWATPVSED VQAPVKLSTL MIVYVALAFG SSLCILLRAT LLVTAGYKTA 
1021:	TELFHKMHHC IFRSPMSFFD STPSGRIMSR ASTDQSAVDL ELPYQFGSVA ITVIQLIGII 
1081:	GVMSQVSWLV FLVFIPVVAA SIWYQRYYIA AARELSRLVG VCKAPLIQHF SETISGATTI 
1141:	RSFSQEFRFR SDNMRLSDGY SRPKFYTAGA MEWLCFRLDM LSSLTFVFSL VFLVSIPTGV 
1201:	IDPSLAGLAV TYGLSLNTLQ AWLIWTLCNL ENKIISVERI LQYASVPSEP PLVIESNRPE 
1261:	QSWPSRGEVE IRDLQVRYAP HMPLVLRGIT CTFKGGLRTG IVGRTGSGKS TLIQTLFRIV 
1321:	EPSAGEIRID GVNILTIGLH DLRLRLSIIP QDPTMFEGTM RSNLDPLEEY TDDQIWEALD 
1381:	KCQLGDEVRK KEQKLDSSVS ENGDNWSMGQ RQLVCLGRVL LKRSKILVLD EATASVDTAT 
1441:	DNLIQKTLRE HFSDCTVITI AHRISSVIDS DMVLLLSNGI IEEYDTPVRL LEDKSSSFSK 
1501:	LVAEYTSRSS SSFD