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3.A.1.208.2
Hepatic canalicular conjugate exporter (the Dubin-Johnson Syndrome protein) (transports bilirubin glucuronides; E2 17 β glucuronide, dianionic bile salts such as taurocholate, taurochenodeoxycholate sulfate and taurolithocholate sulfate; reduced glutathione; glutathione conjugates; glucuronides; cysteinyl leukotrienes; arsenic-glutathione complexes and glutathione disulfide; also exports anthracyclines, epipodophyllotosine, Vinca alkaloids, cisplatin, methotrexate, and the protease inhibitor, lopinavir) (also called ABCC2) (Chen and Tiwari, 2011; Krumpochova et al., 2012).  MK-571 is an inhibitor (Zhang et al., 2011).  Sterol sensing residues have been identified (Gál et al. 2015). Catalyzes efflux of ochratoxin A (OTA) (Qi et al. 2017).

Accession Number:Q92887
Protein Name:MRP2 aka MRP aka cMOAT aka ABCC2 aka cMOAT1 aka cMRP
Length:1545
Molecular Weight:174191.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:16
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Hepatic canalicular conjugates, Bilirubin glucuronides, taurocholate, Taruochenodeoxycholate sulfate, Taurolithocholate sulfate, Reduced glutathione, Glutahione conjugates, Cysteinyl leukotrienes, Arsenic-glutathione complexes, Glutathione dilsulfide, Antracyclines, Epipodophyllotosine, Alkaloids, methotrexate, lopinavir, E2 17 beta glucuronide

Cross database links:

Genevestigator: Q92887
eggNOG: prNOG14927
RefSeq: NP_000383.1   
Entrez Gene ID: 1244   
Pfam: PF00664    PF00005   
Drugbank: Drugbank Link   
OMIM: 237500  phenotype
601107  gene
KEGG: hsa:1244   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0008514 F:organic anion transmembrane transporter act...
GO:0055085 P:transmembrane transport

References (14)

[1] “A human canalicular multispecific organic anion transporter (cMOAT) gene is overexpressed in cisplatin-resistant human cancer cell lines with decreased drug accumulation.”  Taniguchi K.et.al.   8797578
[2] “cDNA cloning of the hepatocyte canalicular isoform of the multidrug resistance protein, cMrp, reveals a novel conjugate export pump deficient in hyperbilirubinemic mutant rats.”  Buechler M.et.al.   8662992
[3] “Exon-intron organization of the human multidrug-resistance protein 2 (MRP2) gene mutated in Dubin-Johnson syndrome.”  Tsujii H.et.al.   10464142
[4] “The DNA sequence and comparative analysis of human chromosome 10.”  Deloukas P.et.al.   15164054
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Hepatic secretion of conjugated drugs and endogenous substances.”  Keppler D.et.al.   11076395
[7] “Mutation of Trp1254 in the multispecific organic anion transporter, multidrug resistance protein 2 (MRP2) (ABCC2), alters substrate specificity and results in loss of methotrexate transport activity.”  Ito K.et.al.   11500505
[8] “Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.”  Daub H.et.al.   18691976
[9] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[10] “Mutations in the canilicular multispecific organic anion transporter (cMOAT) gene, a novel ABC transporter, in patients with hyperbilirubinemia II/Dubin-Johnson syndrome.”  Wada M.et.al.   9425227
[11] “Genomic structure of the canalicular multispecific organic anion-transporter gene (MRP2/cMOAT) and mutations in the ATP-binding-cassette region in Dubin-Johnson syndrome.”  Toh S.et.al.   10053008
[12] “Impaired protein maturation of the conjugate export pump multidrug resistance protein 2 as a consequence of a deletion mutation in Dubin-Johnson syndrome.”  Keitel V.et.al.   11093739
[13] “Identification and functional analysis of two novel mutations in the multidrug resistance protein 2 gene in Israeli patients with Dubin-Johnson syndrome.”  Mor-Cohen R.et.al.   11477083
[14] “Polymorphism of the ABC transporter genes, MDR1, MRP1 and MRP2/cMOAT, in healthy Japanese subjects.”  Ito S.et.al.   11266082

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLEKFCNSTF WNSSFLDSPE ADLPLCFEQT VLVWIPLGFL WLLAPWQLLH VYKSRTKRSS 
61:	TTKLYLAKQV FVGFLLILAA IELALVLTED SGQATVPAVR YTNPSLYLGT WLLVLLIQYS 
121:	RQWCVQKNSW FLSLFWILSI LCGTFQFQTL IRTLLQGDNS NLAYSCLFFI SYGFQILILI 
181:	FSAFSENNES SNNPSSIASF LSSITYSWYD SIILKGYKRP LTLEDVWEVD EEMKTKTLVS 
241:	KFETHMKREL QKARRALQRR QEKSSQQNSG ARLPGLNKNQ SQSQDALVLE DVEKKKKKSG 
301:	TKKDVPKSWL MKALFKTFYM VLLKSFLLKL VNDIFTFVSP QLLKLLISFA SDRDTYLWIG 
361:	YLCAILLFTA ALIQSFCLQC YFQLCFKLGV KVRTAIMASV YKKALTLSNL ARKEYTVGET 
421:	VNLMSVDAQK LMDVTNFMHM LWSSVLQIVL SIFFLWRELG PSVLAGVGVM VLVIPINAIL 
481:	STKSKTIQVK NMKNKDKRLK IMNEILSGIK ILKYFAWEPS FRDQVQNLRK KELKNLLAFS 
541:	QLQCVVIFVF QLTPVLVSVV TFSVYVLVDS NNILDAQKAF TSITLFNILR FPLSMLPMMI 
601:	SSMLQASVST ERLEKYLGGD DLDTSAIRHD CNFDKAMQFS EASFTWEHDS EATVRDVNLD 
661:	IMAGQLVAVI GPVGSGKSSL ISAMLGEMEN VHGHITIKGT TAYVPQQSWI QNGTIKDNIL 
721:	FGTEFNEKRY QQVLEACALL PDLEMLPGGD LAEIGEKGIN LSGGQKQRIS LARATYQNLD 
781:	IYLLDDPLSA VDAHVGKHIF NKVLGPNGLL KGKTRLLVTH SMHFLPQVDE IVVLGNGTIV 
841:	EKGSYSALLA KKGEFAKNLK TFLRHTGPEE EATVHDGSEE EDDDYGLISS VEEIPEDAAS 
901:	ITMRRENSFR RTLSRSSRSN GRHLKSLRNS LKTRNVNSLK EDEELVKGQK LIKKEFIETG 
961:	KVKFSIYLEY LQAIGLFSIF FIILAFVMNS VAFIGSNLWL SAWTSDSKIF NSTDYPASQR 
1021:	DMRVGVYGAL GLAQGIFVFI AHFWSAFGFV HASNILHKQL LNNILRAPMR FFDTTPTGRI 
1081:	VNRFAGDIST VDDTLPQSLR SWITCFLGII STLVMICMAT PVFTIIVIPL GIIYVSVQMF 
1141:	YVSTSRQLRR LDSVTRSPIY SHFSETVSGL PVIRAFEHQQ RFLKHNEVRI DTNQKCVFSW 
1201:	ITSNRWLAIR LELVGNLTVF FSALMMVIYR DTLSGDTVGF VLSNALNITQ TLNWLVRMTS 
1261:	EIETNIVAVE RITEYTKVEN EAPWVTDKRP PPDWPSKGKI QFNNYQVRYR PELDLVLRGI 
1321:	TCDIGSMEKI GVVGRTGAGK SSLTNCLFRI LEAAGGQIII DGVDIASIGL HDLREKLTII 
1381:	PQDPILFSGS LRMNLDPFNN YSDEEIWKAL ELAHLKSFVA SLQLGLSHEV TEAGGNLSIG 
1441:	QRQLLCLGRA LLRKSKILVL DEATAAVDLE TDNLIQTTIQ NEFAHCTVIT IAHRLHTIMD 
1501:	SDKVMVLDNG KIIECGSPEE LLQIPGPFYF MAKEAGIENV NSTKF