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3.A.1.208.20
The vacuolar MRP1 of 1622 aas. Also called ABCC1 and EST1. It has 17 TMSs in a 5 + 6 + 6 arrangement with an N-terminal transmembrane domain of 5 TMSs.  The domain order is N - MCMC, and it transports xenobiotics (Lefèvre and Boutry 2018) It sequesters in the vacuole glutathione conjugates, folate mono-glutamates (pteroyl-1-glutamate) and antifolates (methotrexate); (Raichaudhuri et al. 2009) (86% identical to MRP2 (3.A.1.208.5). ABCC1 and ABCC2 confer tolerance to cadmium and mercury, in addition to their role in arsenic detoxification. MRP1 of Lithospermum erythrorhizon may play a direct or indirect role in transmembrane transport  of shikonin (Zhu et al. 2017). ABCC1 and ABCC2 regulate stomatal closing and opening as well as anthocyanin transport (Frelet-Barrand et al. 2008). ABCC1 of 1622 aas and 17 TMSs in a 5 + 6 + 6 arrangement with an N-terminal transmembrane domain of 5 TMSs.  The domain order is N - MCMC.  Transports xenobiotics (Lefèvre and Boutry 2018)

Accession Number:Q9C8G9
Protein Name:Multidrug resistance-associated protein 1
Length:1622
Molecular Weight:181927.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:15
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Folate mono-glutamates, Antifolates

Cross database links:

Genevestigator: Q9C8G9
eggNOG: KOG0054
HEGENOM: HBG758042
RefSeq: NP_001031116.1    NP_174329.1   
Entrez Gene ID: 839920   
Pfam: PF00664    PF00005   
KEGG: ath:AT1G30400   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0000325 C:plant-type vacuole
GO:0005886 C:plasma membrane
GO:0005774 C:vacuolar membrane
GO:0005524 F:ATP binding
GO:0005516 F:calmodulin binding
GO:0008559 F:xenobiotic-transporting ATPase activity
GO:0055085 P:transmembrane transport

References (13)

[1] “AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene.”  Lu Y.-P.et.al.   9223346
[2] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics.”  Tommasini R.et.al.   9271206
[5] “Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana.”  Sanchez-Fernandez R.et.al.   9671034
[6] “AtMRP2, an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with AtMRP1.”  Lu Y.-P.et.al.   9490749
[7] “The Arabidopsis thaliana ABC protein superfamily, a complete inventory.”  Sanchez-Fernandez R.et.al.   11346655
[8] “Multifunctionality of plant ABC transporters -- more than just detoxifiers.”  Martinoia E.et.al.   11855639
[9] “Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana.”  Kolukisaoglu U.H.et.al.   12430019
[10] “Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters.”  Geisler M.et.al.   15133126
[11] “Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.”  Shimaoka T.et.al.   15215502
[12] “Large-scale characterization of integral proteins from Arabidopsis vacuolar membrane by two-dimensional liquid chromatography.”  Szponarski W.et.al.   14760709
[13] “Plant ABC proteins - a unified nomenclature and updated inventory.”  Verrier P.J.et.al.   18299247

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGFEPLDWYC KPVPNGVWTK TVDYAFGAYT PCAIDSFVLG ISHLVLLILC LYRLWLITKD 
61:	HKVDKFCLRS KWFSYFLALL AAYATAEPLF RLVMRISVLD LDGAGFPPYE AFMLVLEAFA 
121:	WGSALVMTVV ETKTYIHELR WYVRFAVIYA LVGDMVLLNL VLSVKEYYGS FKLYLYISEV 
181:	AVQVAFGTLL FVYFPNLDPY PGYTPVGTEN SEDYEYEELP GGENICPERH ANLFDSIFFS 
241:	WLNPLMTLGS KRPLTEKDVW HLDTWDKTET LMRSFQKSWD KELEKPKPWL LRALNNSLGG 
301:	RFWWGGFWKI GNDCSQFVGP LLLNELLKSM QLNEPAWIGY IYAISIFVGV VLGVLCEAQY 
361:	FQNVMRVGYR LRSALIAAVF RKSLRLTNEG RKKFQTGKIT NLMTTDAESL QQICQSLHTM 
421:	WSAPFRIIVA LVLLYQQLGV ASIIGALFLV LMFPIQTVII SKTQKLTKEG LQRTDKRIGL 
481:	MNEVLAAMDT VKCYAWENSF QSKVQTVRDD ELSWFRKAQL LSAFNMFILN SIPVLVTVVS 
541:	FGVFSLLGGD LTPARAFTSL SLFSVLRFPL FMLPNIITQM VNANVSLNRL EEVLSTEERV 
601:	LLPNPPIEPG QPAISIRNGY FSWDSKADRP TLSNINLDIP LGSLVAVVGS TGEGKTSLIS 
661:	AMLGELPARS DATVTLRGSV AYVPQVSWIF NATVRDNILF GAPFDQEKYE RVIDVTALQH 
721:	DLELLPGGDL TEIGERGVNI SGGQKQRVSM ARAVYSNSDV CILDDPLSAL DAHVGQQVFE 
781:	KCIKRELGQT TRVLVTNQLH FLSQVDKILL VHEGTVKEEG TYEELCHSGP LFQRLMENAG 
841:	KVEDYSEENG EAEVDQTSVK PVENGNANNL QKDGIETKNS KEGNSVLVKR EERETGVVSW 
901:	KVLERYQNAL GGAWVVMMLV ICYVLTQVFR VSSSTWLSEW TDSGTPKTHG PLFYNIVYAL 
961:	LSFGQVSVTL INSYWLIMSS LYAAKKMHDA MLGSILRAPM VFFQTNPLGR IINRFAKDMG 
1021:	DIDRTVAVFV NMFMGSIAQL LSTVILIGIV STLSLWAIMP LLVVFYGAYL YYQNTSREIK 
1081:	RMDSTTRSPV YAQFGEALNG LSSIRAYKAY DRMAEINGRS MDNNIRFTLV NMAANRWLGI 
1141:	RLEVLGGLMV WLTASLAVMQ NGKAANQQAY ASTMGLLLSY ALSITSSLTA VLRLASLAEN 
1201:	SLNSVERVGN YIEIPSEAPL VIENNRPPPG WPSSGSIKFE DVVLRYRPEL PPVLHGVSFL 
1261:	ISPMDKVGIV GRTGAGKSSL LNALFRIVEL EKGRILIDEC DIGRFGLMDL RKVLGIIPQA 
1321:	PVLFSGTVRF NLDPFSEHND ADLWESLERA HLKDTIRRNP LGLDAEVTEA GENFSVGQRQ 
1381:	LLSLARALLR RSKILVLDEA TAAVDVRTDV LIQKTIREEF KSCTMLIIAH RLNTIIDCDK 
1441:	VLVLDSGKVQ EFSSPENLLS NGESSFSKMV QSTGTANAEY LRSITLENKR TREANGDDSQ 
1501:	PLEGQRKWQA SSRWAAAAQF ALAVSLTSSH NDLQSLEIED DNSILKKTKD AVVTLRSVLE 
1561:	GKHDKEIEDS LNQSDISRER WWPSLYKMVE GLAVMSRLAR NRMQHPDYNL EGKSFDWDNV 
1621:	EM