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3.A.1.209.2
Homodimeric transporter ABCB9 or TAPL. Transports a broad spectrum of peptides (low affinity) from the cytosol to the lysosomal lumen. It exists in two forms (812 aas and 1257 aas). The latter full length protein confers resistance to taxanes and anthracyclines (Kawanobe et al., 2012). Resistance and transport were demonstrated for paclitaxel and docetaxel. TapL transports a broad range of peptides of 6-60aas (23aas optimal). It has also been detected in the ER. It is stabilized by interaction with LAMP-1 and LAMP-2 (see 9.A.16) (Demirel et al., 2012).  The protein consists of a core transporter plus an N-terminal transmembrane domain (TMD0) required to tageting to the lysosome and for interactions with LAMP-1 and -2 (Tumulka et al. 2013). TMD0 has a four transmembrane helix topology with a short helical segment in a lysosomal loop (Bock et al. 2018). Lysosomal targeting is determined by membrane localized charged residues (Graab et al. 2019). . 

Accession Number:Q9NP78
Protein Name:ATP-binding cassette sub-family B member 9
Length:766
Molecular Weight:84475.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Lysosome membrane1 / Multi-pass membrane protein2
Substrate peptides, Taxanes, Anthracyclines, Paclitaxel, Docetaxel

Cross database links:

Genevestigator: Q9NP78 Q9NP78
eggNOG: COG1132 COG1132
Entrez Gene ID: 23457   
Pfam: PF00664    PF00005   
KEGG: hsa:23457    hsa:23457   

Gene Ontology

GO:0005765 C:lysosomal membrane
GO:0005886 C:plasma membrane
GO:0042825 C:TAP complex
GO:0005524 F:ATP binding
GO:0042288 F:MHC class I protein binding
GO:0015421 F:oligopeptide-transporting ATPase activity
GO:0042605 F:peptide antigen binding
GO:0042803 F:protein homodimerization activity
GO:0046978 F:TAP1 binding
GO:0046979 F:TAP2 binding
GO:0046980 F:tapasin binding
GO:0001916 P:positive regulation of T cell mediated cytotoxicity
GO:0015031 P:protein transport

References (36)

[1] “A half-type ABC transporter TAPL is highly conserved between rodent and man, and the human gene is not responsive to interferon-gamma in contrast to TAP1 and TAP2.”  Kobayashi A.et.al.   11011155
[2] “Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes.”  Zhang F.et.al.   10748049
[3] “Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity.”  Kobayashi A.et.al.   13679046
[4] “Alternative splice variants encoding unstable protein domains exist in the human brain.”  Homma K.et.al.   15491607
[5] “Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.”  Nagase T.et.al.   10819331
[6] “The finished DNA sequence of human chromosome 12.”  Scherer S.E.et.al.   16541075
[7] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[8] “Membrane localization of transporter associated with antigen processing (TAP)-like (ABCB9) visualized in vivo with a fluorescence protein-fusion technique.”  Kobayashi A.et.al.   15577206
[9] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[10] “Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).”  Wolters J.C.et.al.   15863492
[11] “Identification of a lysosomal peptide transport system induced during dendritic cell development.”  Demirel O.et.al.   17977821
[12] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[13] “Functional dissection of transmembrane domains of human TAP-like (ABCB9).”  Kamakura A.et.al.   18952056
[14] “Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells.”  Ohara T.et.al.   18175933
[15] “Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL).”  Zhao C.et.al.   18434309
[16] “Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain.”  Demirel O.et.al.   20377823
[17] “Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes.”  Fujimoto Y.et.al.   21212514
[18] “Three hundred twenty-six genetic variations in genes encoding nine members of ATP-binding cassette, subfamily B (ABCB/MDR/TAP), in the Japanese population.”  Saito S.et.al.   11829140
[19] “A half-type ABC transporter TAPL is highly conserved between rodent and man, and the human gene is not responsive to interferon-gamma in contrast to TAP1 and TAP2.”  Kobayashi A.et.al.   11011155
[20] “Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes.”  Zhang F.et.al.   10748049
[21] “Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity.”  Kobayashi A.et.al.   13679046
[22] “Alternative splice variants encoding unstable protein domains exist in the human brain.”  Homma K.et.al.   15491607
[23] “Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.”  Nagase T.et.al.   10819331
[24] “The finished DNA sequence of human chromosome 12.”  Scherer S.E.et.al.   16541075
[25] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[26] “Membrane localization of transporter associated with antigen processing (TAP)-like (ABCB9) visualized in vivo with a fluorescence protein-fusion technique.”  Kobayashi A.et.al.   15577206
[27] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[28] “Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).”  Wolters J.C.et.al.   15863492
[29] “Identification of a lysosomal peptide transport system induced during dendritic cell development.”  Demirel O.et.al.   17977821
[30] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[31] “Functional dissection of transmembrane domains of human TAP-like (ABCB9).”  Kamakura A.et.al.   18952056
[32] “Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells.”  Ohara T.et.al.   18175933
[33] “Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL).”  Zhao C.et.al.   18434309
[34] “Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain.”  Demirel O.et.al.   20377823
[35] “Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes.”  Fujimoto Y.et.al.   21212514
[36] “Three hundred twenty-six genetic variations in genes encoding nine members of ATP-binding cassette, subfamily B (ABCB/MDR/TAP), in the Japanese population.”  Saito S.et.al.   11829140

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FASTA formatted sequence
1:	MRLWKAVVVT LAFMSVDICV TTAIYVFSHL DRSLLEDIRH FNIFDSVLDL WAACLYRSCL 
61:	LLGATIGVAK NSALGPRRLR ASWLVITLVC LFVGIYAMVK LLLFSEVRRP IRDPWFWALF 
121:	VWTYISLGAS FLLWWLLSTV RPGTQALEPG AATEAEGFPG SGRPPPEQAS GATLQKLLSY 
181:	TKPDVAFLVA ASFFLIVAAL GETFLPYYTG RAIDGIVIQK SMDQFSTAVV IVCLLAIGSS 
241:	FAAGIRGGIF TLIFARLNIR LRNCLFRSLV SQETSFFDEN RTGDLISRLT SDTTMVSDLV 
301:	SQNINVFLRN TVKVTGVVVF MFSLSWQLSL VTFMGFPIIM MVSNIYGKYY KRLSKEVQNA 
361:	LARASNTAEE TISAMKTVRS FANEEEEAEV YLRKLQQVYK LNRKEAAAYM YYVWGSGLTL 
421:	LVVQVSILYY GGHLVISGQM TSGNLIAFII YEFVLGDCME SVGSVYSGLM QGVGAAEKVF 
481:	EFIDRQPTMV HDGSLAPDHL EGRVDFENVT FTYRTRPHTQ VLQNVSFSLS PGKVTALVGP 
541:	SGSGKSSCVN ILENFYPLEG GRVLLDGKPI SAYDHKYLHR VISLVSQEPV LFARSITDNI 
601:	SYGLPTVPFE MVVEAAQKAN AHGFIMELQD GYSTETGEKG AQLSGGQKQR VAMARALVRN 
661:	PPVLILDEAT SALDAESEYL IQQAIHGNLQ KHTVLIIAHR LSTVEHAHLI VVLDKGRVVQ 
721:	QGTHQQLLAQ GGLYAKLVQR QMLGLQPAAD FTAGHNEPVA NGSHKA