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3.A.1.209.2
Homodimeric transporter ABCB9 or TAPL of 766 aas and 10 TMSs in a 4 + 2 + 2 + 2 TMS arrangement. It transports a broad spectrum of peptides (low affinity) from the cytosol to the lysosomal lumen and displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. It favors positively charged, aromatic and hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored. TapL transports a broad range of peptides of 6-60aas (23aas optimal). It has also been detected in the ER. It is stabilized by interaction with LAMP-1 and LAMP-2 (see 9.A.16) (Demirel et al., 2012).  The protein consists of a core transporter plus an N-terminal transmembrane domain (TMD0) required to tageting to the lysosome and for interactions with LAMP-1 and -2 (Tumulka et al. 2013). TMD0 has a four transmembrane helix topology with a short helical segment in a lysosomal loop (Bock et al. 2018). Lysosomal targeting is determined by membrane localized charged residues (Graab et al. 2019). . 

Accession Number:Q9NP78
Protein Name:ATP-binding cassette sub-family B member 9
Length:766
Molecular Weight:84475.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Lysosome membrane1 / Multi-pass membrane protein2
Substrate peptides, Taxanes, Anthracyclines, Paclitaxel, Docetaxel

Cross database links:

Genevestigator: Q9NP78 Q9NP78
eggNOG: COG1132 COG1132
Entrez Gene ID: 23457   
Pfam: PF00664    PF00005   
KEGG: hsa:23457    hsa:23457   

Gene Ontology

GO:0005765 C:lysosomal membrane
GO:0005886 C:plasma membrane
GO:0042825 C:TAP complex
GO:0005524 F:ATP binding
GO:0042288 F:MHC class I protein binding
GO:0015421 F:oligopeptide-transporting ATPase activity
GO:0042605 F:peptide antigen binding
GO:0042803 F:protein homodimerization activity
GO:0046978 F:TAP1 binding
GO:0046979 F:TAP2 binding
GO:0046980 F:tapasin binding
GO:0001916 P:positive regulation of T cell mediated cytotoxicity
GO:0015031 P:protein transport

References (36)

[1] “A half-type ABC transporter TAPL is highly conserved between rodent and man, and the human gene is not responsive to interferon-gamma in contrast to TAP1 and TAP2.”  Kobayashi A.et.al.   11011155
[2] “Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes.”  Zhang F.et.al.   10748049
[3] “Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity.”  Kobayashi A.et.al.   13679046
[4] “Alternative splice variants encoding unstable protein domains exist in the human brain.”  Homma K.et.al.   15491607
[5] “Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.”  Nagase T.et.al.   10819331
[6] “The finished DNA sequence of human chromosome 12.”  Scherer S.E.et.al.   16541075
[7] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[8] “Membrane localization of transporter associated with antigen processing (TAP)-like (ABCB9) visualized in vivo with a fluorescence protein-fusion technique.”  Kobayashi A.et.al.   15577206
[9] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[10] “Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).”  Wolters J.C.et.al.   15863492
[11] “Identification of a lysosomal peptide transport system induced during dendritic cell development.”  Demirel O.et.al.   17977821
[12] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[13] “Functional dissection of transmembrane domains of human TAP-like (ABCB9).”  Kamakura A.et.al.   18952056
[14] “Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells.”  Ohara T.et.al.   18175933
[15] “Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL).”  Zhao C.et.al.   18434309
[16] “Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain.”  Demirel O.et.al.   20377823
[17] “Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes.”  Fujimoto Y.et.al.   21212514
[18] “Three hundred twenty-six genetic variations in genes encoding nine members of ATP-binding cassette, subfamily B (ABCB/MDR/TAP), in the Japanese population.”  Saito S.et.al.   11829140
[19] “A half-type ABC transporter TAPL is highly conserved between rodent and man, and the human gene is not responsive to interferon-gamma in contrast to TAP1 and TAP2.”  Kobayashi A.et.al.   11011155
[20] “Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes.”  Zhang F.et.al.   10748049
[21] “Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity.”  Kobayashi A.et.al.   13679046
[22] “Alternative splice variants encoding unstable protein domains exist in the human brain.”  Homma K.et.al.   15491607
[23] “Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.”  Nagase T.et.al.   10819331
[24] “The finished DNA sequence of human chromosome 12.”  Scherer S.E.et.al.   16541075
[25] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[26] “Membrane localization of transporter associated with antigen processing (TAP)-like (ABCB9) visualized in vivo with a fluorescence protein-fusion technique.”  Kobayashi A.et.al.   15577206
[27] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[28] “Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).”  Wolters J.C.et.al.   15863492
[29] “Identification of a lysosomal peptide transport system induced during dendritic cell development.”  Demirel O.et.al.   17977821
[30] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[31] “Functional dissection of transmembrane domains of human TAP-like (ABCB9).”  Kamakura A.et.al.   18952056
[32] “Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells.”  Ohara T.et.al.   18175933
[33] “Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL).”  Zhao C.et.al.   18434309
[34] “Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain.”  Demirel O.et.al.   20377823
[35] “Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes.”  Fujimoto Y.et.al.   21212514
[36] “Three hundred twenty-six genetic variations in genes encoding nine members of ATP-binding cassette, subfamily B (ABCB/MDR/TAP), in the Japanese population.”  Saito S.et.al.   11829140

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRLWKAVVVT LAFMSVDICV TTAIYVFSHL DRSLLEDIRH FNIFDSVLDL WAACLYRSCL 
61:	LLGATIGVAK NSALGPRRLR ASWLVITLVC LFVGIYAMVK LLLFSEVRRP IRDPWFWALF 
121:	VWTYISLGAS FLLWWLLSTV RPGTQALEPG AATEAEGFPG SGRPPPEQAS GATLQKLLSY 
181:	TKPDVAFLVA ASFFLIVAAL GETFLPYYTG RAIDGIVIQK SMDQFSTAVV IVCLLAIGSS 
241:	FAAGIRGGIF TLIFARLNIR LRNCLFRSLV SQETSFFDEN RTGDLISRLT SDTTMVSDLV 
301:	SQNINVFLRN TVKVTGVVVF MFSLSWQLSL VTFMGFPIIM MVSNIYGKYY KRLSKEVQNA 
361:	LARASNTAEE TISAMKTVRS FANEEEEAEV YLRKLQQVYK LNRKEAAAYM YYVWGSGLTL 
421:	LVVQVSILYY GGHLVISGQM TSGNLIAFII YEFVLGDCME SVGSVYSGLM QGVGAAEKVF 
481:	EFIDRQPTMV HDGSLAPDHL EGRVDFENVT FTYRTRPHTQ VLQNVSFSLS PGKVTALVGP 
541:	SGSGKSSCVN ILENFYPLEG GRVLLDGKPI SAYDHKYLHR VISLVSQEPV LFARSITDNI 
601:	SYGLPTVPFE MVVEAAQKAN AHGFIMELQD GYSTETGEKG AQLSGGQKQR VAMARALVRN 
661:	PPVLILDEAT SALDAESEYL IQQAIHGNLQ KHTVLIIAHR LSTVEHAHLI VVLDKGRVVQ 
721:	QGTHQQLLAQ GGLYAKLVQR QMLGLQPAAD FTAGHNEPVA NGSHKA