TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.3.1.4
Non-gastric H+-, K+- or NH4+-ATPase (Swarts et al., 2005; Worrell et al., 2008)

Accession Number:P54708
Protein Name:Atp12a aka Atp1al1
Length:1036
Molecular Weight:114975.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate H+, K+, NH4+

Cross database links:

Genevestigator: P54708
eggNOG: maNOG09271
RefSeq: NP_598201.1   
Entrez Gene ID: 171028   
Pfam: PF00689    PF00690    PF00122    PF00702   
KEGG: rno:171028   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0008900 F:hydrogen:potassium-exchanging ATPase activity
GO:0046872 F:metal ion binding
GO:0006754 P:ATP biosynthetic process
GO:0006813 P:potassium ion transport
GO:0015992 P:proton transport
GO:0010038 P:response to metal ion
GO:0014070 P:response to organic cyclic substance

References (3)

[1] “Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus.”  Crowson M.S.et.al.   1320029
[2] “A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia.”  Kone B.C.et.al.   9446555
[3] “Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit.”  Pestov N.B.et.al.   9872395

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK ELDLDDHRLS 
61:	NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP EIIKFLKQMV GGFSILLWIG 
121:	AALCWIAFVI QYVNNSASLD NVYLGAILVL VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ 
181:	ALVIRDAEKK VISAEQLVVG DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS 
241:	TEFTHENPLE TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI 
301:	EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA TVTVTLSLTA 
361:	KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV AHLWFDNQIF VADTSENQTK 
421:	QAFDQSSGTW ASLSKIITLC NRAEFRPGQE SVPIMKRTVV GDASETALLK FSEVILGDVM 
481:	GIRKRNHKVA EIPFNSTNKF QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ 
541:	PLDKSSADSF HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL 
601:	LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN ETVEDIAKRR 
661:	NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI VFARTSPQQK LIIVEGCQRQ 
721:	DAIVAVTGDG VNDSPALKKA DIGIAMGIAG SDAAKNAADM VLLDDNFASI VTGVEEGRLI 
781:	FDNLKKTIAY TLTKNIAELC PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE 
841:	SDIMNRKPRH KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV 
901:	AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK TRRNSIFQQG 
961:	LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY WFVAVPHAIL IWVYDEMRKL 
1021:	FIRLYPGSWW DKNMYY