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3.A.3.2.1
Plasma membrane Ca2+-ATPase (efflux), PMCA4 (Giacomello et al. 2013).  The CD147 immunosupression protein interacts via its immunomodulatory domains with PMCA4 to bypass T-cell receptor proximal signaling and inhibit interleukin-2 (IL-2) expression (Supper et al. 2016). Deletion of residues 300 - 349, corresponding the the residues deleted in a natural splice variant (de Tezanos Pinto and Adamo 2006).

Accession Number:P23634
Protein Name:ATP2B4
Length:1241
Molecular Weight:137920.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Ca2+

Cross database links:

Genevestigator: P23634
DIP: DIP-6128N
RefSeq: NP_001001396.1    NP_001675.3   
Entrez Gene ID: 493   
Pfam: PF12424    PF00689    PF00690    PF00122    PF00702   
OMIM: 108732  gene
KEGG: hsa:493   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005524 F:ATP binding
GO:0005388 F:calcium-transporting ATPase activity
GO:0005516 F:calmodulin binding
GO:0046872 F:metal ion binding
GO:0005515 F:protein binding
GO:0006754 P:ATP biosynthetic process
GO:0006816 P:calcium ion transport

References (10)

[1] “Peptide sequence analysis and molecular cloning reveal two calcium pump isoforms in the human erythrocyte membrane.”  Strehler E.E.et.al.   2137451
[2] “Analysis of the tissue-specific distribution of mRNAs encoding the plasma membrane calcium-pumping ATPases and characterization of an alternately spliced form of PMCA4 at the cDNA and genomic levels.”  Brandt P.et.al.   1531651
[3] “The full-ORF clone resource of the German cDNA consortium.”  Bechtel S.et.al.   17974005
[4] “The DNA sequence and biological annotation of human chromosome 1.”  Gregory S.G.et.al.   16710414
[5] “Analysis of mRNA expression and cloning of a novel plasma membrane Ca(2+)-ATPase splice variant in human heart.”  Santiago-Garcia J.et.al.   8700162
[6] “Identification and primary structure of a calmodulin binding domain of the Ca2+ pump of human erythrocytes.”  James P.et.al.   2963820
[7] “A C-terminal, calmodulin-like regulatory domain from the plasma membrane Ca2+-pumping ATPase.”  Brandt P.et.al.   2966397
[8] “Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes.”  Stauffer T.P.et.al.   8245032
[9] “”  Stauffer T.P.et.al.   7989379
[10] “NMR solution structure of a complex of calmodulin with a binding peptide of the Ca(2+) pump.”  Elshorst B.et.al.   10493800
Structure:
1CFF   2KNE     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 
61:	SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 
121:	FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 
181:	QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 
241:	GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 
301:	GKKQGVPENR NKAKTQDGVA LEIQPLNSQE GIDNEEKDKK AVKVPKKEKS VLQGKLTRLA 
361:	VQIGKAGLLM SALTVFILIL YFVIDNFVIN RRPWLPECTP IYIQYFVKFF IIGITVLVVA 
421:	VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 
481:	YIGGIHYRQI PSPDVFLPKV LDLIVNGISI NSAYTSKILP PEKEGGLPRQ VGNKTECALL 
541:	GFVTDLKQDY QAVRNEVPEE KLYKVYTFNS VRKSMSTVIR NPNGGFRMYS KGASEIILRK 
601:	CNRILDRKGE AVPFKNKDRD DMVRTVIEPM ACDGLRTICI AYRDFDDTEP SWDNENEILT 
661:	ELTCIAVVGI EDPVRPEVPD AIAKCKQAGI TVRMVTGDNI NTARAIATKC GILTPGDDFL 
721:	CLEGKEFNRL IRNEKGEVEQ EKLDKIWPKL RVLARSSPTD KHTLVKGIID STVGEHRQVV 
781:	AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 
841:	SKFLQFQLTV NVVAVIVAFT GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTESLL 
901:	KRRPYGRNKP LISRTMMKNI LGHAFYQLIV IFILVFAGEK FFDIDSGRKA PLHSPPSQHY 
961:	TIVFNTFVLM QLFNEINSRK IHGEKNVFSG IYRNIIFCSV VLGTFICQIF IVEFGGKPFS 
1021:	CTSLSLSQWL WCLFIGIGEL LWGQFISAIP TRSLKFLKEA GHGTTKEEIT KDAEGLDEID 
1081:	HAEMELRRGQ ILWFRGLNRI QTQIDVINTF QTGASFKGVL RRQNMGQHLD VKLVPSSSYI 
1141:	KVVKAFHSSL HESIQKPYNQ KSIHSFMTHP EFAIEEELPR TPLLDEEEEE NPDKASKFGT 
1201:	RVLLLDGEVT PYANTNNNAV DCNQVQLPQS DSSLQSLETS V