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3.A.3.3.1
H+-ATPase (efflux)

Accession Number:P07038
Protein Name:PMA1 aka PMA-1 aka B1D1.210
Length:920
Molecular Weight:99887.00
Species:Neurospora crassa [5141]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

eggNOG: fuNOG06156
RefSeq: XP_956886.1   
Entrez Gene ID: 3873048   
Pfam: PF00690    PF00122    PF00702   
BioCyc: NCRA-XX3-01:NCRA-XX3-01-005390-MONOMER   
KEGG: ncr:NCU01680   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0008553 F:hydrogen-exporting ATPase activity, phospho...
GO:0046872 F:metal ion binding
GO:0006754 P:ATP biosynthetic process
GO:0015992 P:proton transport

References (9)

[1] “Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences.”  Hager K.M.et.al.   2876429
[2] “Primary structure of the Neurospora plasma membrane H+-ATPase deduced from the gene sequence. Homology to Na+/K+-, Ca2+-, and K+-ATPase.”  Addison R.et.al.   2876992
[3] “What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence.”  Mannhaupt G.et.al.   12655011
[4] “The genome sequence of the filamentous fungus Neurospora crassa.”  Galagan J.E.et.al.   12712197
[5] “Cysteine 532 and cysteine 545 are the N-ethylmaleimide-reactive residues of the Neurospora plasma membrane H+-ATPase.”  Pardo J.P.et.al.   2524486
[6] “Identification of the major cytoplasmic regions of the Neurospora crassa plasma membrane H(+)-ATPase using protein chemical techniques.”  Scarborough G.A.et.al.   2144525
[7] “Identification of the membrane-embedded regions of the Neurospora crassa plasma membrane H(+)-ATPase.”  Rao U.S.et.al.   1830591
[8] “Cytoplasmic location of amino acids 359-440 of the Neurospora crassa plasma membrane H(+)-ATPase.”  Rao U.S.et.al.   1386255
[9] “Topology of the Neurospora plasma membrane H(+)-ATPase. Localization of a transmembrane segment.”  Lin A.et.al.   8106434
Structure:
1MHS     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MADHSASGAP ALSTNIESGK FDEKAAEAAA YQPKPKVEDD EDEDIDALIE DLESHDGHDA 
61:	EEEEEEATPG GGRVVPEDML QTDTRVGLTS EEVVQRRRKY GLNQMKEEKE NHFLKFLGFF 
121:	VGPIQFVMEG AAVLAAGLED WVDFGVICGL LLLNAVVGFV QEFQAGSIVD ELKKTLALKA 
181:	VVLRDGTLKE IEAPEVVPGD ILQVEEGTII PADGRIVTDD AFLQVDQSAL TGESLAVDKH 
241:	KGDQVFASSA VKRGEAFVVI TATGDNTFVG RAAALVNAAS GGSGHFTEVL NGIGTILLIL 
301:	VIFTLLIVWV SSFYRSNPIV QILEFTLAIT IIGVPVGLPA VVTTTMAVGA AYLAKKKAIV 
361:	QKLSAIESLA GVEILCSDKT GTLTKNKLSL HDPYTVAGVD PEDLMLTACL AASRKKKGID 
421:	AIDKAFLKSL KYYPRAKSVL SKYKVLQFHP FDPVSKKVVA VVESPQGERI TCVKGAPLFV 
481:	LKTVEEDHPI PEEVDQAYKN KVAEFATRGF RSLGVARKRG EGSWEILGIM PCMDPPRHDT 
541:	YKTVCEAKTL GLSIKMLTGD AVGIARETSR QLGLGTNIYN AERLGLGGGG DMPGSEVYDF 
601:	VEAADGFAEV FPQHKYNVVE ILQQRGYLVA MTGDGVNDAP SLKKADTGIA VEGSSDAARS 
661:	AADIVFLAPG LGAIIDALKT SRQIFHRMYA YVVYRIALSI HLEIFLGLWI AILNRSLNIE 
721:	LVVFIAIFAD VATLAIAYDN APYSQTPVKW NLPKLWGMSV LLGVVLAVGT WITVTTMYAQ 
781:	GENGGIVQNF GNMDEVLFLQ ISLTENWLIF ITRANGPFWS SIPSWQLSGA IFLVDILATC 
841:	FTIWGWFEHS DTSIVAVVRI WIFSFGIFCI MGGVYYILQD SVGFDNLMHG KSPKGNQKQR 
901:	SLEDFVVSLQ RVSTQHEKSQ