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3.A.3.3.7
Plasma membrane H+ ATPase, AHA1 Three isoforms, AHA1, 2 & 3, exhibit different kinetic properties (Palmgren and Christensen, 1994). Both the N- and C-termini are directly involved in controlling the pump activity (Ekberg et al., 2010). Methyl jasmonate elicits stomatal closure in many plant species including A. thaliana, and stomatal closure is accompanied by large ion fluxes across the plasma membrane.  These events appear to be mediated by AHA1 (Yan et al. 2015).

Accession Number:P20649
Protein Name:ATPase 1, plasma membrane-type aka AHA1
Length:949
Molecular Weight:104224.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

Genevestigator: P20649
eggNOG: KOG0205
HEGENOM: HBG706356
RefSeq: NP_179486.1   
Entrez Gene ID: 816413   
Pfam: PF00690    PF00122    PF00702   
KEGG: ath:AT2G18960   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005634 C:nucleus
GO:0005886 C:plasma membrane
GO:0005773 C:vacuole
GO:0005524 F:ATP binding
GO:0008553 F:hydrogen-exporting ATPase activity, phospho...
GO:0046872 F:metal ion binding
GO:0005515 F:protein binding
GO:0006754 P:ATP biosynthetic process
GO:0015992 P:proton transport
GO:0010119 P:regulation of stomatal movement
GO:0009737 P:response to abscisic acid stimulus
GO:0009414 P:response to water deprivation

References (10)

[1] “Molecular cloning and sequence of cDNA encoding the plasma membrane proton pump (H+-ATPase) of Arabidopsis thaliana.”  Harper J.F.et.al.   2521951
[2] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.”  Nuehse T.S.et.al.   15308754
[5] “Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis.”  Niittylae T.et.al.   17586839
[6] “Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.”  Benschop J.J.et.al.   17317660
[7] “Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses.”  Nuehse T.S.et.al.   17651370
[8] “Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis.”  de la Fuente van Bentem S.et.al.   18433157
[9] “Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana.”  Jones A.M.E.et.al.   19245862
[10] “Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.”  Reiland S.et.al.   19376835

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSGLEDIKNE TVDLEKIPIE EVFQQLKCTR EGLTTQEGED RIVIFGPNKL EEKKESKILK 
61:	FLGFMWNPLS WVMEAAALMA IALANGDNRP PDWQDFVGII CLLVINSTIS FIEENNAGNA 
121:	AAALMAGLAP KTKVLRDGKW SEQEAAILVP GDIVSIKLGD IIPADARLLE GDPLKVDQSA 
181:	LTGESLPVTK HPGQEVFSGS TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL 
241:	TSIGNFCICS IAIGIAIEIV VMYPIQHRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG 
301:	SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC KGVEKDQVLL 
361:	FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF NPVDKRTALT YIDSDGNWHR 
421:	VSKGAPEQIL DLANARPDLR KKVLSCIDKY AERGLRSLAV ARQVVPEKTK ESPGGPWEFV 
481:	GLLPLFDPPR HDSAETIRRA LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSAALLGT 
541:	DKDSNIASIP VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD 
601:	IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV SITIRIVFGF 
661:	MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT PDSWKLKEIF ATGIVLGGYQ 
721:	AIMSVIFFWA AHKTDFFSDK FGVRSIRDNN DELMGAVYLQ VSIISQALIF VTRSRSWSFV 
781:	ERPGALLMIA FVIAQLVATL IAVYADWTFA KVKGIGWGWA GVIWIYSIVT YFPQDILKFA 
841:	IRYILSGKAW ASLFDNRTAF TTKKDYGIGE REAQWAQAQR TLHGLQPKED VNIFPEKGSY 
901:	RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVAKLKGLDI DTAGHHYTV