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3.A.3.3.9
Proton pumping ATPase, AHA2.  94% identical to AHA1 (3.A.3.3.7); generates the plasma membrane pmf.  Cation-binding pockets have been identified (Ekberg et al. 2010).  The pump has been reconstituted into "nanodiscs" in a functionally monomeric form (Justesen et al. 2013).  Regulated at the post-translation level by cis-acting auto-inhibitory domains, which can be relieved by protein kinase-mediated phosphorylation or binding of specific lipid species such as lysophospholipids (Wielandt et al. 2015).  Pumping is stochastically interrupted by long-lived (~100 seconds) inactive or leaky states. Allosteric regulation by pH gradients modulates the switch between these states but not the pumping or leakage rates (Veshaguri et al. 2016).  They dynamics of the pump have been examined (Guerra and Bondar 2015). AHA2 drives root cell expansion (Hoffmann et al. 2018).

Accession Number:P19456
Protein Name:ATPase 2, plasma membrane-type
Length:948
Molecular Weight:104401.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

Structure:
3B8C   5ksd     

External Searches:

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  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSLEDIKNE TVDLEKIPIE EVFQQLKCSR EGLTTQEGED RIQIFGPNKL EEKKESKLLK 
61:	FLGFMWNPLS WVMEMAAIMA IALANGDGRP PDWQDFVGII CLLVINSTIS FIEENNAGNA 
121:	AAALMAGLAP KTKVLRDGKW SEQEAAILVP GDIVSIKLGD IIPADARLLE GDPLKVDQSA 
181:	LTGESLPVTK HPGQEVFSGS TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL 
241:	TAIGNFCICS IAIGMVIEII VMYPIQRRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG 
301:	SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC KGVEKDQVLL 
361:	FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF NPVDKRTALT YIDGSGNWHR 
421:	VSKGAPEQIL ELAKASNDLS KKVLSIIDKY AERGLRSLAV ARQVVPEKTK ESPGAPWEFV 
481:	GLLPLFDPPR HDSAETIRRA LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSSALLGT 
541:	HKDANLASIP VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD 
601:	IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV SITIRIVFGF 
661:	MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT PDSWKLKEIF ATGVVLGGYQ 
721:	AIMTVIFFWA AHKTDFFSDT FGVRSIRDNN HELMGAVYLQ VSIISQALIF VTRSRSWSFV 
781:	ERPGALLMIA FLIAQLIATL IAVYANWEFA KIRGIGWGWA GVIWLYSIVT YFPLDVFKFA 
841:	IRYILSGKAW LNLFENKTAF TMKKDYGKEE REAQWALAQR TLHGLQPKEA VNIFPEKGSY 
901:	RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVVKLKGLDI ETPSHYTV