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3.A.3.5.38
Cuprous ion (Cu+) exporter, CopB, of 785 aas and 8 TMSs in a 4 + 2 + 2 arrangement. The copper-transporting P1B-ATPases have been divided traditionally into two subfamilies, the P1B-1-ATPases or CopAs and the P1B-3-ATPases or CopBs. CopAs selectively export Cu+ whereas previous studies have suggested that CopBs are specific for Cu2+ export. Biochemical and spectroscopic characterization of Sphaerobacter thermophilus CopB (StCopB) showed that, while it does bind Cu2+, the binding site is not in the transmembrane domain (Purohit et al. 2018).  StCopB exhibits metal-stimulated ATPase activity in response to Cu+, but not Cu2+, indicating that it is actually a Cu+ transporter. Cu+ is coordinated by four sulfur ligands derived from conserved cysteine and methionine residues. The histidine-rich N-terminal region is required for maximal activity, but is inhibitory in the presence of divalent metal ions. P1B-1- and P1B-3-ATPases may therefore all transport Cu+ (Purohit et al. 2018).

Accession Number:D1CA99
Protein Name:Copper-translocating P-type ATPase
Length:785
Molecular Weight:83208.00
Species:Sphaerobacter thermophilus (strain DSM 20745 / S 6022) [479434]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell membrane1
Substrate

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FASTA formatted sequence
1:	MSSTPQHPEH HRRGHGNHAA HPESGHTEHT EHVDHAGHPE HREHTDHADH DHAERMASVE 
61:	GRAGELEHAA HTTHAGHAGH DAHEAHAEHS GHADHPAHGG HAGHGGHAGH EGHADVFRRR 
121:	FWINLILTIP ILLYSEMLQD WLGFSMPVFP GSDLIPPVLG TVVFFYGGAV FILGGWDEIR 
181:	TRQPGMMLLI SMAIVVAFVA SAASTLGWFN LEFWWELALL IDVMLLGHWL EMRALGQAQG 
241:	ALQALAALLP DEAERVVDGT VERVPLDSLR PGDIVLVRPG ARVPADGTIV DGRAELDESM 
301:	LTGESRPVPK EIGDPVVAGS VAQGSALRVR VDAVGADTAL AGIERLVTEA QQSRSRAQAL 
361:	ADRAAALLFY VAVVAGLATF IIWTVLGDID SGVVRTVTVL VISCPHALGL AIPLVIALST 
421:	SLSARQGILV RDRLALERMR EITTVLFDKT GTLTRGEHAV TGIVALDDDA DALLAEAAAV 
481:	EADSEHPLAR AIVTAAEARR VSVPRAKDFR AIAGRGVEAQ VDGVEVAVGG PNLLRERGLE 
541:	VPDALAERVR PWQERGAAVL HVVRDGRVVG ALALEDEVRP ESREAVEALH ARGIRVVMIT 
601:	GDARPVAEAV GAELGVDEVF AEVLPEDKAN AVATLQQRGE RVAMVGDGVN DAPALARADV 
661:	GIAIGAGTDV AIESAGVILA SNDPRGVVSV MELSRASYRK MVENLVWAAG YNIVAIPLAA 
721:	GVLAPWGIVL PPAVGALLMS ASTIIVALNA QTLRRLDLRP EAATHEHGGP PPTRGRREPA 
781:	LSRAG