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3.A.3.6.18
Cd2+/Zn2+ exporting ATPase, HMA4. (very similar to HMA3; TC# 3.A.3.6.7). Important for Zn2+ nutrition. Has a C-terminal domain containing 13 cysteine pairs and a terminal stretch of 11 histidines with a high affinity for Zn2+ and Cd2+ and a capacity to bind 10 Zn2+ ions per C-terminus (Baekgaard et al., 2010). The pathway of translocatioin through the protein has been investigated, and the demonstration that mutations affect Zn2+ and Cd2+ transport differentially has been reported (Lekeux et al. 2018).

Accession Number:O64474
Protein Name:Putative cadmium/zinc-transporting ATPase 2
Length:1172
Molecular Weight:127209.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Zn2+, Cd2+

Cross database links:

Genevestigator: O64474
eggNOG: KOG0207
HEGENOM: HBG507745
Entrez Gene ID: 816428   
Pfam: PF00122    PF00702   
KEGG: ath:AT2G19110   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0008551 F:cadmium-exporting ATPase activity
GO:0004008 F:copper-exporting ATPase activity
GO:0046872 F:metal ion binding
GO:0005515 F:protein binding
GO:0016463 F:zinc-exporting ATPase activity
GO:0006754 P:ATP biosynthetic process
GO:0046686 P:response to cadmium ion
GO:0032025 P:response to cobalt ion
GO:0010043 P:response to zinc ion
GO:0055069 P:zinc ion homeostasis

References (3)

[1] “Functional expression of AtHMA4, a P1B-type ATPase of the Zn/Co/Cd/Pb subclass.”  Mills R.F.et.al.   12848823
[2] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
Structure:
2KKH     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MALQNKEEEK KKVKKLQKSY FDVLGICCTS EVPIIENILK SLDGVKEYSV IVPSRTVIVV 
61:	HDSLLISPFQ IAKALNEARL EANVRVNGET SFKNKWPSPF AVVSGLLLLL SFLKFVYSPL 
121:	RWLAVAAVAA GIYPILAKAF ASIKRPRIDI NILVIITVIA TLAMQDFMEA AAVVFLFTIS 
181:	DWLETRASYK ATSVMQSLMS LAPQKAIIAE TGEEVEVDEV KVDTVVAVKA GETIPIDGIV 
241:	VDGNCEVDEK TLTGEAFPVP KQRDSTVWAG TINLNGYICV KTTSLAGDCV VAKMAKLVEE 
301:	AQSSKTKSQR LIDKCSQYYT PAIILVSACV AIVPVIMKVH NLKHWFHLAL VVLVSGCPCG 
361:	LILSTPVATF CALTKAATSG LLIKSADYLD TLSKIKIVAF DKTGTITRGE FIVIDFKSLS 
421:	RDINLRSLLY WVSSVESKSS HPMAATIVDY AKSVSVEPRP EEVEDYQNFP GEGIYGKIDG 
481:	NDIFIGNKKI ASRAGCSTVP EIEVDTKGGK TVGYVYVGER LAGFFNLSDA CRSGVSQAMA 
541:	ELKSLGIKTA MLTGDNQAAA MHAQEQLGNV LDVVHGDLLP EDKSRIIQEF KKEGPTAMVG 
601:	DGVNDAPALA TADIGISMGI SGSALATQTG NIILMSNDIR RIPQAVKLAR RARRKVVENV 
661:	CLSIILKAGI LALAFAGHPL IWAAVLVDVG TCLLVIFNSM LLLREKKKIG NKKCYRASTS 
721:	KLNGRKLEGD DDYVVDLEAG LLTKSGNGQC KSSCCGDKKN QENVVMMKPS SKTSSDHSHP 
781:	GCCGDKKEEK VKPLVKDGCC SEKTRKSEGD MVSLSSCKKS SHVKHDLKMK GGSGCCASKN 
841:	EKGKEVVAKS CCEKPKQQVE SVGDCKSGHC EKKKQAEDIV VPVQIIGHAL THVEIELQTK 
901:	ETCKTSCCDS KEKVKETGLL LSSENTPYLE KGVLIKDEGN CKSGSENMGT VKQSCHEKGC 
961:	SDEKQTGEIT LASEEETDDQ DCSSGCCVNE GTVKQSFDEK KHSVLVEKEG LDMETGFCCD 
1021:	AKLVCCGNTE GEVKEQCRLE IKKEEHCKSG CCGEEIQTGE ITLVSEEETE STNCSTGCCV 
1081:	DKEEVTQTCH EKPASLVVSG LEVKKDEHCE SSHRAVKVET CCKVKIPEAC ASKCRDRAKR 
1141:	HSGKSCCRSY AKELCSHRHH HHHHHHHHHV SA