TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.3.6.2
Zn2+-, Cd2+-, Co2+-, Hg2+-, Ni2+-, Cu2+, Pb2+-ATPase (efflux) (Hou and Mitra, 2003)

Accession Number:P37617
Protein Name:ATZN aka ZntA aka B3469
Length:732
Molecular Weight:76840.00
Species:Escherichia coli [83333]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Pb2+, Cu2+, Ni2+, Hg2+, Co2+, Cd2+, Zn2+

Cross database links:

Genevestigator: P37617
EchoBASE: EB2129
EcoGene: EG12215
eggNOG: COG2217
HEGENOM: HBG507745
DIP: DIP-12947N
RefSeq: AP_004323.1    NP_417926.1   
Entrez Gene ID: 947972   
Pfam: PF00122    PF00403    PF00702   
BioCyc: EcoCyc:YHHO-MONOMER    ECOL168927:B3469-MONOMER   
KEGG: ecj:JW3434    eco:b3469   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0008551 F:cadmium-exporting ATPase activity
GO:0046872 F:metal ion binding
GO:0016463 F:zinc-exporting ATPase activity
GO:0006754 P:ATP biosynthetic process
GO:0010312 P:detoxification of zinc ion
GO:0030001 P:metal ion transport
GO:0046686 P:response to cadmium ion

References (8)

[1] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase.”  Rensing C.et.al.   9405611
[5] “Zinc(II) tolerance in Escherichia coli K-12: evidence that the zntA gene (o732) encodes a cation transport ATPase.”  Beard S.J.et.al.   9364914
[6] “The ATP hydrolytic activity of purified ZntA, a Pb(II)/Cd(II)/Zn(II)-translocating ATPase from Escherichia coli.”  Sharma R.et.al.   10660539
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[8] “A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118).”  Banci L.et.al.   12417201
Structure:
1MWY   1MWZ   4umv     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MSTPDNHGKK APQFAAFKPL TTVQNANDCC CDGACSSTPT LSENVSGTRY SWKVSGMDCA 
61:	ACARKVENAV RQLAGVNQVQ VLFATEKLVV DADNDIRAQV ESALQKAGYS LRDEQAAEEP 
121:	QASRLKENLP LITLIVMMAI SWGLEQFNHP FGQLAFIATT LVGLYPIARQ ALRLIKSGSY 
181:	FAIETLMSVA AIGALFIGAT AEAAMVLLLF LIGERLEGWA ASRARQGVSA LMALKPETAT 
241:	RLRKGEREEV AINSLRPGDV IEVAAGGRLP ADGKLLSPFA SFDESALTGE SIPVERATGD 
301:	KVPAGATSVD RLVTLEVLSE PGASAIDRIL KLIEEAEERR APIERFIDRF SRIYTPAIMA 
361:	VALLVTLVPP LLFAASWQEW IYKGLTLLLI GCPCALVIST PAAITSGLAA AARRGALIKG 
421:	GAALEQLGRV TQVAFDKTGT LTVGKPRVTA IHPATGISES ELLTLAAAVE QGATHPLAQA 
481:	IVREAQVAEL AIPTAESQRA LVGSGIEAQV NGERVLICAA GKHPADAFTG LINELESAGQ 
541:	TVVLVVRNDD VLGVIALQDT LRADAATAIS ELNALGVKGV ILTGDNPRAA AAIAGELGLE 
601:	FKAGLLPEDK VKAVTELNQH APLAMVGDGI NDAPAMKAAA IGIAMGSGTD VALETADAAL 
661:	THNHLRGLVQ MIELARATHA NIRQNITIAL GLKGIFLVTT LLGMTGLWLA VLADTGATVL 
721:	VTANALRLLR RR