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3.A.3.8.10
Lipid flippase, Apt1 (involved in stress tolerance and virulence). Deletion of Apt1 causes (1) altered actin distribution, (2) increased sensitivity to stress conditions (oxidative and nitrosative stress) and to trafficking inhibitors, such as brefeldin A and monensin, a reduction in exported acid phosphatase activity, and (3) hypersensitivity to the antifungal drugs amphotericin B, fluconazole, and cinnamycin (Hu and Kronstad, 2010).

Accession Number:Q5KP96
Protein Name:Calcium transporting ATPase, putative
Length:1326
Molecular Weight:148977.00
Species:cryptococcus neoformans [5207]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Endosome membrane1 / Multi-pass membrane protein2
Substrate lipids

Cross database links:

HEGENOM: HBG745019
RefSeq: XP_566703.1   
Entrez Gene ID: 3253992   
Pfam: PF00122    PF00702   
KEGG: cne:CNA03720   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0015662 F:ATPase activity, coupled to transmembrane m...
GO:0000287 F:magnesium ion binding
GO:0004012 F:phospholipid-translocating ATPase activity
GO:0006754 P:ATP biosynthetic process
GO:0015914 P:phospholipid transport

References (1)

[1] “The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.”  Loftus B.J.et.al.   15653466

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MPAQQQDPFD DLLGSDSPPA PPRNDIFHEG RHIDFFHQGS STARAPAAIS QRGSSPDSVN 
61:	QATYALDPFF DDDDEYGPAV SNSYSGYLAP HPSSALHSSQ PLGRSDPSLL ESSMPLANAG 
121:	AMPAGFSGPM DDNDVKGYQA SSSTAYAAED PFRDDEGPSA YAFTAPGASS GWTQPRRSRW 
181:	QMFRDDYLTD VDWSFGVNQL LRRRSKFDGV PREIALNEPE ENRLKGFERN SVTTGKYGPI 
241:	TFLPKFLLSE FSRSANLFFL FTACIQQVPN VSPTGHWTTI VPLGVVIIAS AFKEIKEDFK 
301:	RHASDRSLNN NLAQVLVDQQ FQLRPWRRLR VGDIVRLEAN SFIPADIVLI SSSEPEGLCY 
361:	VETANLDGET NLKIKQAHPS TASLTNPHSV SLLRGHILSE PPNSSLYTYD GTFHLSSAHP 
421:	GSAPTKIPVG PNQMLLRGAQ LRNTGWVYGV IVNAGHETKL MRNATEAPVK RTAVERQVNR 
481:	QILYLFLLLI VLSLVSTIGS SIRTWLFDKN AWYLRLGDES KNKARQFIED ILTFIILYNN 
541:	LIPISLIMTM EVVKFQQASL INSDLDMYYA PTDTPALCRT SSLVEELGQI AYIFSDKTGT 
601:	LTRNEMEFRE CTIFGTMYAQ TVDDNKRDQG QKTFDSLRHR AQEDSQEGHV IREFLSLLSI 
661:	CHTVIPEEHD GKMVYQASSP DEAALVAGAE MLGYRFQTRK PKSVFIDVNG ETQEWEILNV 
721:	CEFNSSRKRM STVVRGPDGT IKLYTKGADT VIFERLAPKQ EFSEPTLVHL EDYATEGLRT 
781:	LCLAYRDISE EEYSSWSALY NNAASQMSGR AEALDKAAEV IEQNLQLLGA TAVEDKLQDG 
841:	VPDAIHTLQQ AGIKIWVLTG DRQETAINIG LSCRLISESM NLVIVNTETA VETSELLNKR 
901:	LFAIKNQRLG GDTEELALII DGKSLTYALE KDCSDVFLEL AIMCKAVICC RVSPLQKALV 
961:	VKLVKRSTDA PLLAIGDGAN DVSMIQAAHV GVGISGVEGL QAARSADIAI SQFRFLRKLL 
1021:	LVHGSWSYQR LTKLILYSFY KNITFALTLF WYSWFNDYSG QIAFEGWSMS YYNVVFTILP 
1081:	PLVIGIFDQF VSARMLDRYP QLYHLGQQNY FFTPIRFFYW VGNAFYHSVL LFAFSVLVFY 
1141:	NDLLATDGKN SGLWVWGTTL YLAVLLTVLG KAALISDVWT KYTLAAIPGS FIFTMIALPL 
1201:	YAIIAPLLNF SLEYTGIVPR LWADPVFYFV LLLFPIICLL RDYVWKYYRR TYHPASYHIV 
1261:	QEIQKFSLSD YRPRQEQFQK AIKKVRATQR MRRQRGFAFS QTETNNQDQA RLIRAYDTSV 
1321:	ARPSGY