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3.A.3.8.5
Inwardly directed phosphatidylcholine, phosphatidyl serine, and lysophosphoethanolamine flippase, Dnf2 (functions with the β-subunit, Lem3) (Elvington et al., 2005; Pomorski et al., 2003; Riekhof and Voelker, 2006; Riekhof et al., 2007). This plasma membrane P-type ATPase (ACT4) is a phospholipid flippase that contributes to endocytosis, protein transport and all polarity (Hua et al., 2002). Transports monoacyl (lyso) phospholipids much better than diacyl phospholipids, but can be mutated to transport diacyl phospholipids (Baldridge et al. 2013).

Accession Number:Q12675
Protein Name:Dnf2 aka Ydr093W aka Yd8557.01
Length:1612
Molecular Weight:182619.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Phosphatidylcholine, phosphatidyl serine, Lysophosphoethanolamine

Cross database links:

Genevestigator: Q12675
eggNOG: fuNOG04167
HEGENOM: HBG745019
DIP: DIP-8055N
RefSeq: NP_010378.1   
Entrez Gene ID: 851667   
Pfam: PF00122    PF00702   
KEGG: sce:YDR093W   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005624 C:membrane fraction
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0015662 F:ATPase activity, coupled to transmembrane m...
GO:0000287 F:magnesium ion binding
GO:0004012 F:phospholipid-translocating ATPase activity
GO:0005515 F:protein binding
GO:0006754 P:ATP biosynthetic process
GO:0006897 P:endocytosis
GO:0007163 P:establishment or maintenance of cell polarity
GO:0006886 P:intracellular protein transport
GO:0045332 P:phospholipid translocation

References (6)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[2] “An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system.”  Hua Z.et.al.   12221123
[3] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[4] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[5] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[6] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
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  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSPSKPTSP FVDDIEHESG SASNGLSSMS PFDDSFQFEK PSSAHGNIEV AKTGGSVLKR 
61:	QSKPMKDIST PDLSKVTFDG IDDYSNDNDI NDDDELNGKK TEIHEHENEV DDDLHSFQAT 
121:	PMPNTGGFED VELDNNEGSN NDSQADHKLK RVRFGTRRNK SGRIDINRSK TLKWAKKNFH 
181:	NAIDEFSTKE DSLENSALQN RSDELRTVYY NLPLPEDMLD EDGLPLAVYP RNKIRTTKYT 
241:	PLTFFPKNIL FQFHNFANIY FLILLILGAF QIFGVTNPGF ASVPLIVIVI ITAIKDGIED 
301:	SRRTVLDLEV NNTRTHILSG VKNENVAVDN VSLWRRFKKA NTRALIKIFE YFSENLTAAG 
361:	REKKLQKKRE ELRRKRNSRS FGPRGSLDSI GSYRMSADFG RPSLDYENLN QTMSQANRYN 
421:	DGENLVDRTL QPNPECRFAK DYWKNVKVGD IVRVHNNDEI PADMILLSTS DVDGACYVET 
481:	KNLDGETNLK VRQSLKCSKI IKSSRDITRT KFWVESEGPH ANLYSYQGNF KWQDTQNGNI 
541:	RNEPVNINNL LLRGCTLRNT KWAMGMVIFT GDDTKIMINA GVTPTKKSRI SRELNFSVIL 
601:	NFVLLFILCF TAGIVNGVYY KQKPRSRDYF EFGTIGGSAS TNGFVSFWVA VILYQSLVPI 
661:	SLYISVEIIK TAQAIFIYTD VLLYNAKLDY PCTPKSWNIS DDLGQIEYIF SDKTGTLTQN 
721:	VMEFKKCTIN GVSYGRAYTE ALAGLRKRQG VDVESEGRRE KEEIAKDRET MIDELRSMSD 
781:	NTQFCPEDLT FVSKEIVEDL KGSSGDHQQK CCEHFLLALA LCHSVLVEPN KDDPKKLDIK 
841:	AQSPDESALV STARQLGYSF VGSSKSGLIV EIQGVQKEFQ VLNVLEFNSS RKRMSCIIKI 
901:	PGSTPKDEPK ALLICKGADS VIYSRLDRTQ NDATLLEKTA LHLEEYATEG LRTLCLAQRE 
961:	LTWSEYERWV KTYDVAAASV TNREEELDKV TDVIERELIL LGGTAIEDRL QDGVPDSIAL 
1021:	LAEAGIKLWV LTGDKVETAI NIGFSCNVLN NDMELLVVKA SGEDVEEFGS DPIQVVNNLV 
1081:	TKYLREKFGM SGSEEELKEA KREHGLPQGN FAVIIDGDAL KVALNGEEMR RKFLLLCKNC 
1141:	KAVLCCRVSP AQKAAVVKLV KKTLDVMTLA IGDGSNDVAM IQSADVGVGI AGEEGRQAVM 
1201:	CSDYAIGQFR YVTRLVLVHG KWCYKRLAEM IPQFFYKNVI FTLSLFWYGI YNNFDGSYLF 
1261:	EYTYLTFYNL AFTSVPVILL AVLDQDVSDT VSMLVPQLYR VGILRKEWNQ TKFLWYMLDG 
1321:	VYQSVICFFF PYLAYHKNMV VTENGLGLDH RYFVGVFVTA IAVTSCNFYV FMEQYRWDWF 
1381:	CGLFICLSLA VFYGWTGIWT SSSSSNEFYK GAARVFAQPA YWAVLFVGVL FCLLPRFTID 
1441:	CIRKIFYPKD IEIVREMWLR GDFDLYPQGY DPTDPSRPRI NEIRPLTDFK EPISLDTHFD 
1501:	GVSHSQETIV TEEIPMSILN GEQGSRKGYR VSTTLERRDQ LSPVTTTNNL PRRSMASARG 
1561:	NKLRTSLDRT REEMLANHQL DTRYSVERAR ASLDLPGINH AETLLSQRSR DR