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3.A.3.8.8
The phosphatidylserine flippase in photoreceptor disc membranes, ATP8A2 (Coleman et al., 2009). The beta-subunit, CDC50A (TC#8.A.27.1.5), allows the stable expression, assembly, subcellular localization, and lipid transport activity of ATP8A2 (Coleman and Molday, 2011).  Missennse mutations in ATP8A2 are associated with cerebellar atrophy and guadrupedal locomotion (Emre Onat et al. 2012). Asparagine-905 of the mammalian phospholipid flippase ATP8A2 is essential for lipid substrate-induced activation of ATP8A2 dephosphorylation (Mikkelsen et al. 2019).

Accession Number:P98200
Protein Name:Probable phospholipid-transporting ATPase IB
Length:1148
Molecular Weight:129417.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate phosphatidyl serine

Cross database links:

Genevestigator: P98200
eggNOG: roNOG14737
HEGENOM: HBG745019
RefSeq: NP_056618.1   
Entrez Gene ID: 50769   
Pfam: PF00122   
KEGG: mmu:50769   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0015662 F:ATPase activity, coupled to transmembrane m...
GO:0000287 F:magnesium ion binding
GO:0004012 F:phospholipid-translocating ATPase activity
GO:0006754 P:ATP biosynthetic process
GO:0015914 P:phospholipid transport

References (3)

[1] “Differential expression of putative transbilayer amphipath transporters.”  Halleck M.S.et.al.   11015572
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.”  Munton R.P.et.al.   17114649

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSRATSVGDQ LEAPARIIYL NQSHLNKFCD NRISTAKYSV LTFLPRFLYE QIRRAANAFF 
61:	LFIALLQQIP DVSPTGRYTT LVPLVIILTI AGIKEIIEDF KRHKADNAVN KKKTIVLRNG 
121:	MWHTIMWKEV AVGDIVKVLN GQYLPADMVL FSSSEPQGMC YVETANLDGE TNLKIRQGLS 
181:	HTTDMQTRDV LMKLSGRIEC EGPNRHLYDF TGNLHLDGKS SVALGPDQIL LRGTQLRNTQ 
241:	WVFGVVVYTG HDSKLMQNST KAPLKRSNVE KVTNVQILVL FGILLVMALV SSVGALFWNG 
301:	SHGGKSWYIK KMDTNSDNFG YNLLTFIILY NNLIPISLLV TLEVVKYTQA LFINWDMDMY 
361:	YIENDTPAMA RTSNLNEELG QVKYLFSDKT GTLTCNIMNF KKCSIAGVTY GHFPELAREQ 
421:	SSDDFCRMTS CTNDSCDFND PRLLKNIEDQ HPTAPCIQEF LTLLAVCHTV VPEKDGDEII 
481:	YQASSPDEAA LVKGAKKLGF VFTGRTPYSV IIEAMGQEQT FGILNVLEFS SDRKRMSVIV 
541:	RLPSGQLRLY CKGADNVIFE RLSKDSKYME ETLCHLEYFA TEGLRTLCVA YADLSENEYE 
601:	EWLKVYQEAS IILKDRAQRL EECYEIIEKN LLLLGATAIE DRLQAGVPET IATLLKAEIK 
661:	IWVLTGDKQE TAINIGYSCR LVSQNMALIL LKEDSLDATR AAITQHCTDL GNLLGKENDV 
721:	ALIIDGHTLK YALSFEVRRS FLDLALSCKA VICCRVSPLQ KSEIVDVVKK RVKAITLAIG 
781:	DGANDVGMIQ TAHVGVGISG NEGMQATNNS DYAIAQFSYL EKLLLVHGAW SYNRVTKCIL 
841:	YCFYKNVVLY IIELWFAFVN GFSGQILFER WCIGLYNVIF TALPPFTLGI FERSCTQESM 
901:	LRFPQLYRIT QNAEGFNTKV FWGHCINALV HSLILFWVPM KALEHDTPVT SGHATDYLFV 
961:	GNIVYTYVVV TVCLKAGLET TAWTKFSHLA VWGSMLIWLV FFGVYSTIWP TIPIAPDMKG 
1021:	QATMVLSSAY FWLGLFLVPT ACLIEDVAWR AAKHTCKKTL LEEVQELETK SRVMGKAMLR 
1081:	DSNGKRMNER DRLIKRLSRK TPPTLFRTGS IQQCVSHGYA FSQEEHGAVT QEEIVRAYDT 
1141:	TKENSRKK