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3.E.1.1.6
Sensory rhodopsin (green-light-activated photoreceptor; does not transport ions) (Jung et al., 2003). Has all-trans-retinal when dark adapted, but 11-cis-retinal when light adapted due to reversible interconversion (Sineshchekov et al., 2005).  Anabaena sensory rhodopsin, a photochromic sensor that interacts with a soluble 14-kDa cytoplasmic transducer that is encoded on the same operon, interconverts between all-trans-15-anti and 13-cis-15-syn retinal forms depending on the wavelength of illumination, although only the former participates in a photocycle with a signaling M intermediate (Dong et al. 2016). A mutation in the cytoplasmic half-channel of the protein, replacing Asp217 with Glu (D217E), results in the creation of a light-driven, single- photon, inward proton transporter. Dong et al. 2016 presented the 2.3 A structure of dark-adapted D217E ASR, which reveals changes in the water network surrounding Glu217, as well as a shift in the carbon backbone near retinal-binding Lys210, illustrating a possible pathway leading to the protonation of Glu217 in the cytoplasmic half-channel, located 15 A from the Schiff base.  

Accession Number:Q8YSC4
Protein Name:ALR3165
Length:261
Molecular Weight:30191.00
Species:Anabaena sp. (strain PCC 7120) [103690]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

eggNOG: COG5524
HEGENOM: HBG440674
RefSeq: NP_487205.1   
Entrez Gene ID: 1106763   
Pfam: PF01036   
BioCyc: NSP103690:ALR3165-MONOMER   
KEGG: ana:alr3165   

Gene Ontology

GO:0016020 C:membrane
GO:0005216 F:ion channel activity
GO:0006811 P:ion transport

References (2)

[1] “Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120.”  Kaneko T.et.al.   11759840
[2] “Solution structure of the carboxyl-terminal cysteine-rich domain of the VHv1.1 polydnaviral gene product: comparison with other cystine knot structural folds.”  Einerwold J.et.al.   11724552
Structure:
1XIO   2M3G   4TL3     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNLESLLHWI YVAGMTIGAL HFWSLSRNPR GVPQYEYLVA MFIPIWSGLA YMAMAIDQGK 
61:	VEAAGQIAHY ARYIDWMVTT PLLLLSLSWT AMQFIKKDWT LIGFLMSTQI VVITSGLIAD 
121:	LSERDWVRYL WYICGVCAFL IILWGIWNPL RAKTRTQSSE LANLYDKLVT YFTVLWIGYP 
181:	IVWIIGPSGF GWINQTIDTF LFCLLPFFSK VGFSFLDLHG LRNLNDSRQT TGDRFAENTL 
241:	QFVENITLFA NSRRQQSRRR V