PEP-dependent, phosphoryl transfer-driven group translocators of the bacterial phosphoenolpyruvate:sugar phosphotransferase system are the best characterized group translocators included in TC category 4. The product of the reaction, derived from extracellular sugar, is a cytoplasmic sugar-phosphate. The enzymatic constituents, catalyzing sugar phosphorylation, are superimposed on the transport process in a tightly coupled process. The PTS is also involved in regulation and chemotaxis (Barabote & Saier et al., 2005;     Lengeler & Jahreis et al., 2009).