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8.A.24.1.2
Solute carrier family 9, subfamily A (NHE3 cation proton antiporter 3 regulatory factor), member 3 regulator 2, SLC9A3R2, SNX27 (sorting nexin 27) or NHERF2 of 337 aas.  It binds erzin and affects dexamethasone stimulated NHE3 activity (Yang et al. 2015).  It also regulates several other transporters including CFTR (TC# 3.A.1.202.1) (Zhang et al. 2017). SNX27 directly interacts with DRA (SLC26A3; TC# 2.A.53.2.18) in early endosomes and mediates its direct recycling to facilitate high activity in lipid rafts in the apical plasma membrane (Bannert et al. 2020).

Accession Number:Q15599
Protein Name:Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Length:337
Molecular Weight:37414.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Endomembrane system1 / Peripheral membrane protein2
Substrate NONE

Cross database links:

Genevestigator: Q15599 Q15599
eggNOG: NOG319972 NOG319972
HEGENOM: HOG000089940 HOG000089940
DIP: DIP-29093N DIP-29093N
Entrez Gene ID: 9351   
Pfam: PF09007    PF00595   
KEGG: hsa:9351    hsa:9351   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0012505 C:endomembrane system
GO:0005634 C:nucleus
GO:0008013 F:beta-catenin binding
GO:0019902 F:phosphatase binding
GO:0008022 F:protein C-terminus binding
GO:0005102 F:receptor binding
GO:0006461 P:protein complex assembly
GO:0005737 C:cytoplasm
GO:0005886 C:plasma membrane
GO:0016247 F:channel regulator activity
GO:0048839 P:inner ear development
GO:0014067 P:negative regulation of phosphatidylinositol 3-kinase cascade
GO:0045838 P:positive regulation of membrane potential

References (32)

[1] “The human testis determining factor SRY binds a nuclear factor containing PDZ protein interaction domains.”  Poulat F.et.al.   9054412
[2] “Identification of EBP50: a PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family.”  Reczek D.et.al.   9314537
[3] “cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.”  Yun C.H.C.et.al.   9096337
[4] “Genomic structure and sequence of a human homologue (NTHL1/NTH1) of Escherichia coli endonuclease III with those of the adjacent parts of TSC2 and SLC9A3R2 genes.”  Imai K.et.al.   9831664
[5] “A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins.”  Hall R.A.et.al.   9671706
[6] “The sequence and analysis of duplication-rich human chromosome 16.”  Martin J.et.al.   15616553
[7] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[8] “cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor.”  Zizak M.et.al.   10455146
[9] “Molecular requirements for the regulation of the renal outer medullary K(+) channel ROMK1 by the serum- and glucocorticoid-inducible kinase SGK1.”  Palmada M.et.al.   14623317
[10] “NHERF2 specifically interacts with LPA2 receptor and defines the specificity and efficiency of receptor-mediated phospholipase C-beta3 activation.”  Oh Y.-S.et.al.   15143197
[11] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[12] “Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells.”  Meder D.et.al.   15642748
[13] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[14] “MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells.”  Arnaud C.et.al.   19555689
[15] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460
[16] “Structural basis for NHERF recognition by ERM proteins.”  Terawaki S.et.al.   16615918
[17] “The human testis determining factor SRY binds a nuclear factor containing PDZ protein interaction domains.”  Poulat F.et.al.   9054412
[18] “Identification of EBP50: a PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family.”  Reczek D.et.al.   9314537
[19] “cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.”  Yun C.H.C.et.al.   9096337
[20] “Genomic structure and sequence of a human homologue (NTHL1/NTH1) of Escherichia coli endonuclease III with those of the adjacent parts of TSC2 and SLC9A3R2 genes.”  Imai K.et.al.   9831664
[21] “A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins.”  Hall R.A.et.al.   9671706
[22] “The sequence and analysis of duplication-rich human chromosome 16.”  Martin J.et.al.   15616553
[23] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[24] “cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor.”  Zizak M.et.al.   10455146
[25] “Molecular requirements for the regulation of the renal outer medullary K(+) channel ROMK1 by the serum- and glucocorticoid-inducible kinase SGK1.”  Palmada M.et.al.   14623317
[26] “NHERF2 specifically interacts with LPA2 receptor and defines the specificity and efficiency of receptor-mediated phospholipase C-beta3 activation.”  Oh Y.-S.et.al.   15143197
[27] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[28] “Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells.”  Meder D.et.al.   15642748
[29] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[30] “MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells.”  Arnaud C.et.al.   19555689
[31] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460
[32] “Structural basis for NHERF recognition by ERM proteins.”  Terawaki S.et.al.   16615918
Structure:
2D11   2HE4   2OCS   4P0C     

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FASTA formatted sequence
1:	MAAPEPLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL RAGDRLVEVN 
61:	GVNVEGETHH QVVQRIKAVE GQTRLLVVDQ ETDEELRRRQ LTCTEEMAQR GLPPAHDPWE 
121:	PKPDWAHTGS HSSEAGKKDV SGPLRELRPR LCHLRKGPQG YGFNLHSDKS RPGQYIRSVD 
181:	PGSPAARSGL RAQDRLIEVN GQNVEGLRHA EVVASIKARE DEARLLVVDP ETDEHFKRLR 
241:	VTPTEEHVEG PLPSPVTNGT SPAQLNGGSA CSSRSDLPGS DKDTEDGSAW KQDPFQESGL 
301:	HLSPTAAEAK EKARAMRVNK RAPQMDWNRK REIFSNF