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9.A.14.3.2
The muscarinic acetylcholine G-protein-coupled receptor (Jo et al., 2010). Endothelial-dependent muscarinic receptor signaling acts largely through TRPV4 sparklet-mediated stimulation of IK (1.A.1.16.2) and SK (1.A.1.16.1) channels to promote vasodilation. There are five muscarinic receptor subtypes (M1R to M5R), which, despite sharing a high degree of sequence identity in the transmembrane region, couple to different heterotrimeric G proteins to transmit signals. M1R, M3R, and M5R couple to the Gq/ 11 family, whereas M2R and M4R couple to the Gi/ o family. Maeda et al. 2019 presented and compared the cryo-electron microscopy structures of M1R in complex with G11 and M2R in complex with GoA. The M1R-G11 complex exhibits distinct features, including an extended transmembrane helix 5 and carboxyl-terminal receptor tail that interacts with the G protein. Detailed analysis provides a framework for understanding the molecular determinants of G-protein coupling selectivity (Maeda et al. 2019).

Accession Number:P12657
Protein Name:Muscarinic acetylcholine receptor M1
Length:460
Molecular Weight:51329.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate NONE

Cross database links:

Entrez Gene ID: 12669   
Pfam: PF00001   
KEGG: mmu:12669    mmu:12669   

Gene Ontology

GO:0030054 C:cell junction
GO:0016021 C:integral to membrane
GO:0045211 C:postsynaptic membrane
GO:0040012 P:regulation of locomotion
GO:0005886 C:plasma membrane
GO:0016907 F:G-protein coupled acetylcholine receptor activity

References (4)

[1] “Isolation, sequence, and functional expression of the mouse M1 muscarinic acetylcholine receptor gene.”  Shapiro R.A.et.al.   2848036
[2] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
[3] “Isolation, sequence, and functional expression of the mouse M1 muscarinic acetylcholine receptor gene.”  Shapiro R.A.et.al.   2848036
[4] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
Structure:
5cxv     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNTSVPPAVS PNITVLAPGK GPWQVAFIGS TTGLLSLATV TGNLLVLISI KVNTELKTVN 
61:	NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 
121:	FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 
181:	QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 
241:	SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 
301:	VIKMPMVDPE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE 
361:	KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STVNPMCYAS 
421:	CNKAFRDHFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC