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9.B.17.1.1
Vesicle-associated membrane protein-associated protein-B/C, VAMP-B/C or VAPB). It has 243 aas with one C-terminal TMS and the large N-terminal domain extracellular. It binds to several organelle-resident membrane proteins to mediate ER-organelle tethering. Mutations give rise to amyotrophic lateral sclerosis (ALS) because the mutant proteins induce protein misfolding and aggregation, leading to ER disorganization. Thus, altered ER domain properties and impaired ER-organelle tethering result (Yamanaka et al. 2020).

Accession Number:O95292
Protein Name:VAP-B/C
Length:243
Molecular Weight:27228.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass type IV membrane protein2
Substrate

Cross database links:

RefSeq: NP_004729.1   
Entrez Gene ID: 9217   
Pfam: PF00635   
OMIM: 182980  phenotype
605704  gene
608627  phenotype
KEGG: hsa:9217   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0005794 C:Golgi apparatus
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0048487 F:beta-tubulin binding
GO:0019899 F:enzyme binding
GO:0046982 F:protein heterodimerization activity
GO:0042803 F:protein homodimerization activity
GO:0005198 F:structural molecule activity
GO:0008219 P:cell death
GO:0030968 P:endoplasmic reticulum unfolded protein resp...
GO:0045070 P:positive regulation of viral genome replica...
GO:0019048 P:virus-host interaction

References (14)

[1] “Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins.”  Nishimura Y.et.al.   9920726
[2] “Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.”  Hu R.-M.et.al.   10931946
[3] “The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.”  Clark H.F.et.al.   12975309
[4] “The DNA sequence and comparative analysis of human chromosome 20.”  Deloukas P.et.al.   11780052
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.”  Gevaert K.et.al.   12665801
[7] “Human VAP-B is involved in hepatitis C virus replication through interaction with NS5A and NS5B.”  Hamamoto I.et.al.   16227268
[8] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[9] “Phosphoproteome analysis of the human mitotic spindle.”  Nousiainen M.et.al.   16565220
[10] “Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis.”  Wang B.et.al.   19007248
[11] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[12] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[13] “Lysine acetylation targets protein complexes and co-regulates major cellular functions.”  Choudhary C.et.al.   19608861
[14] “A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis.”  Nishimura A.L.et.al.   15372378
Structure:
2MDK   3IKK     

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Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR RYCVRPNSGI 
61:	IDAGASINVS VMLQPFDYDP NEKSKHKFMV QSMFAPTDTS DMEAVWKEAK PEDLMDSKLR 
121:	CVFELPAEND KPHDVEINKI ISTTASKTET PIVSKSLSSS LDDTEVKKVM EECKRLQGEV 
181:	QRLREENKQF KEEDGLRMRK TVQSNSPISA LAPTGKEEGL STRLLALVVL FFIVGVIIGK 
241:	IAL