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9.B.231.1.1
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC 2.4.1.256) (Alpha-1,2-glucosyltransferase ALG10-A) (Alpha-2-glucosyltransferase ALG10) (Asparagine-linked glycosylation protein 10) (Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10)

Accession Number:P50076
Protein Name:Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Length:525
Molecular Weight:61793.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

Genevestigator: P50076
eggNOG: NOG236252
HEGENOM: HOG000240902
Entrez Gene ID: 853142   
Pfam: PF04922   
KEGG: sce:YGR227W   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0016021 C:integral to membrane
GO:0004583 F:dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity
GO:0006488 P:dolichol-linked oligosaccharide biosynthetic process

References (4)

[1] “Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae.”  Nikawa J.et.al.   7565092
[2] “Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII.”  van der Aart Q.J.M.et.al.   8701610
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[4] “The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation.”  Burda P.et.al.   9597543

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDAKKNTGEA NNDVLEEEAA IQLIAPGIAR NLTQEVITGI FCNVVIYPLL LIYFVLTFRY 
61:	MTTNIVPYEF IDEKFHVGQT LTYLKGKWTQ WDPKITTPPG IYILGLINYY CIKPIFKSWS 
121:	TLTILRLVNL LGGIIVFPIL VLRPIFLFNA LGFWPVSLMS FPLMTTYYYL FYTDVWSTIL 
181:	ILQSLSCVLT LPFGPVKSIW LSAFFAGVSC LFRQTNIIWT GFIMILAVER PAILQKQFNT 
241:	HTFNNYLKLF IHAIDDFSNL VLPYMINFVL FFIYLIWNRS ITLGDKSSHS AGLHIVQIFY 
301:	CFTFITVFSL PIWISRNFMK LYKLRIKRKP VQTFFEFIGI MLIIRYFTKV HPFLLADNRH 
361:	YTFYLFRRLI GNKSRLIKYF FMTPIYHFST FAYLEVMRPN QLTFHPITPL PIKEPVHLPI 
421:	QLTHVSWTAL ITCTMVTIVP SPLFEPRYYI LPYFFWRIFI TCSCEPLIKD LKPAKEGENP 
481:	ITISSTKRLF MEFLWFMLFN VVTLVIFSKV SFPWTTEPYL QRIIW