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9.B.36.1.1
The acid resistance 6 TMS HdeD protein of 190 aas. The yhiD and hdeD gene products participate in an acid resistance mechanism exhibited only at high cell densities (Mates et al. 2007). H-NS directly controls expression of the hedD gene (Krin et al. 2010).  Both small RNAs, CyaR and RprA, interact with the 5'-UTR of hdeD mRNA, and binding to the RBS of hdeD, interfers with translational initiation. Lalaouna et al. 2018 identified an A/U-rich motif on hdeD, which is bound by the RNA chaperone Hfq. The results indicate that binding of this motif by Hfq is required for CyaR-induced degradation of hdeD mRNA, and two molecules of RprA must bind the 5'-UTR of hdeD to block translation initiation. While both CyaR and RprA sRNAs bind to the same motif on the hdeD mRNA, RprA solely acts at the translational level, leaving the target RNA intact (Lalaouna et al. 2018).

Accession Number:P0AET5
Protein Name:HdeD aka b3511 aka JW3479
Length:190
Molecular Weight:20903.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Unknown

Cross database links:

Genevestigator: P0AET5
EchoBASE: EB1458
EcoGene: EG11495
eggNOG: COG3247
HEGENOM: HBG636791
DIP: DIP-48170N
RefSeq: AP_004282.1    NP_417968.1   
Entrez Gene ID: 948024   
BioCyc: EcoCyc:EG11495-MONOMER    ECOL168927:B3511-MONOMER   
KEGG: ecj:JW3479    eco:b3511   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0009268 P:response to pH

References (6)

[1] “Physical map location of a set of Escherichia coli genes (hde) whose expression is affected by the nucleoid protein H-NS.”  Yoshida T.et.al.   8244952
[2] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Function of the Escherichia coli nucleoid protein, H-NS: molecular analysis of a subset of proteins whose expression is enhanced in a hns deletion mutant.”  Yoshida T.et.al.   8455549
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

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Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLYIDKATIL KFDLEMLKKH RRAIQFIAVL LFIVGLLCIS FPFVSGDILS TVVGALLICS 
61:	GIALIVGLFS NRSHNFWPVL SGFLVAVAYL LIGYFFIRAP ELGIFAIAAF IAGLFCVAGV 
121:	IRLMSWYRQR SMKGSWLQLV IGVLDIVIAW IFLGATPMVS VTLVSTLVGI ELIFSAASLF 
181:	SFASLFVKQQ