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9.B.39 The Long Chain Fatty Acid Translocase (lcFAT) Family

The CD36 antigen, also called platelet glycoprotein IV (GPIV) and the PAS-4 protein (PASIV), has been implicated in the uptake of long chain fatty acids in mouse tissues such as heart, skeletal muscle and adipose tissue (Coburn et al., 2000). The mouse protein, of 472 aas, exhibits two hydrophobic segments that may be TMSs, one at its extreme N-terminus, and one at its extreme C-terminus.

Leptin has been shown to increase fatty acid oxidation and intramuscular triacylglycerol hydrolysis. Chronic leptin administration decreases fatty acid uptake and reduces mRNA levels of FAT/CD36 in rat skeletal muscle (Steinberg et al., 2002). The plasma membrane-associated fatty acid binding protein (FABPpm), also implicated in fatty acid transport, was also expressed at reduced levels following leptin treatment. It acts as a fatty acid sink once fatty acids have crossed the plasma membrane.

The reaction believed to be catalyzed by CD36 is:

long chain fatty acid (out) Æ long chain fatty acid (in)

References associated with 9.B.39 family:

Bartosch, B., A. Vitelli, C. Granier, C. Goujon, J. Dubuisson, S. Pascale, E. Scarselli, R. Cortese, A. Nicosia, and F.L. Cosset. (2003). Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor. J. Biol. Chem. 278: 41624-41630. 12913001
Coburn, C.T., F.F. Knapp, Jr., M. Febraio, A.L. Beets, R.L. Silverstein and N. Abumrad (2000). Defective uptake and utilization of long chain fatty acids in muscle and adipose tissues of CD36 knockout mice. J. Biol. Chem. 275: 32523-32529. 10913136
Sakudoh, T., T. Iizuka, J. Narukawa, H. Sezutsu, I. Kobayashi, S. Kuwazaki, Y. Banno, A. Kitamura, H. Sugiyama, N. Takada, H. Fujimoto, K. Kadono-Okuda, K. Mita, T. Tamura, K. Yamamoto, and K. Tsuchida. (2010). A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration. J. Biol. Chem. 285: 7739-7751. 20053988
Steinberg, G.R., D.J. Dyck, J. Calles-Escandon, N.N. Tandon, J.J.F.P. Luiken, J.F.C. Glatz, and A. Bonen. (2002). Chronic leptin administration decreases fatty acid uptake and fatty acid transporters in rat skeletal muscle. J. Biol. Chem. 277: 8854-8860. 11729182