2.A.135.1.1
Heterodimeric inorganic cation antiporter, UmpAB, both subunits belonging to the DUF1538 family. Genomic DNA was screened for novel Na+/H+ antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na+/H+ antiporters (Meng et al. 2017). Co-expression of two genes designated umpAB, encoding
paired homologous unknown membrane proteins belonging to DUF1538 family, were found to confer E. coli KNabc tolerance to 0.4 M NaCl and 30 mM LiCl, and an alkaline pH
resistance at 8.0. UmpAB localizes as a hetero-dimer in the cytoplasmic membrane and exhibits pH-dependent Na+(Li+, K+)/H+ antiport activity at a wide pH range of 6.5 to 9.5 with an optimal pH
at 9.0. Neither UmpA nor UmpB showed homology with known single-gene or
multi-gene Na+/H+ antiporters, and thus, UmpAB represents a novel two-component Na+(Li+, K+)/H+ antiporter (Meng et al. 2017).
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| Accession Number: | A0A1X9QDU4.1 |
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| Protein Name: | UMPA_HALZH RecName: Full=Na(+), Li(+), K(+)/H(+) antiporter subunit A; AltName: Full=Na(+) (Li(+)/K(+))/H(+) antiporter subunit A |
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| Length: | 255 |
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| Molecular Weight: | |
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| Species: | Halomonas zhaodongensis [1176240] |
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| Number of TMSs: | 7 |
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| Substrate |
lithium(1+), sodium(1+), potassium(1+), hydron |
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1: MNIVYSLANQ LKDSLRDLLP VVLVMGFFQL VVIRQPLPDS LALLDLVLGL VFVVVGLTLF
61: IKGLELGLFP LGENLARAFV KKGSVAWLLI FAFALGFGST VAEPALIAVA ARAGEVVSES
121: GLIADTSAAQ ASFAFTLRMV VALSVGTAVV VGVLRIFKGW PLHLMIIAGY IIVLLLTLFA
181: PNELIGIAYD SGGVTTSTIT VPLVTALGVG LASTIKGRNP MLDGFGLIAF ASVMPIIFVL
241: LFGIFGLSLV TGST