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1.E.31.1.14
Two component holin, requiring two non-homologous small proteins, one of 87 aas and 2 TMSs (E0U1U5), a member of family TC# 1.E.31, and another gene product, XhlA, of 89 aas with 1 C-terminal TMS (P39798), not homologous to members of family 1.E.31 (Fernandes and São-José 2017).

Accession Number:E0U1U5
Protein Name:Bacteriophage PBSX holin
Length:87
Molecular Weight:9776.00
Species:Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) [655816]
Number of TMSs:2
Substrate

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKMFDKGTVI RTVLLLVALI NQTMLMLGKS PLDIQEEQVN QLADALYSAG SVIFTIGTTL 
61:	AAWFKNNYVT EKGKKQRDLL KENNLTK