4.D.3.1.4
Trimeric cellulose synthase coomplex for primary cell wall synthesis, CESA1, 3 and 6 (Watanabe et al. 2015). CESA1 is of 1084 aas and 8 TMSs while other CESA subunits are of similar size. All three are catalytic
subunit of the cellulose synthase complex. Required
for beta-1,4-glucan microfibril crystallization, a major mechanism of primary cell wall formation. Required during embryogenesis for cell elongation, orientation of cell
expansion and complex cell wall formation (Bashline et al. 2014).
determined the structure of a poplar cellulose synthase CesA homotrimer
that suggests a molecular basis for cellulose microfibril formation.
This complex, stabilized by cytosolic plant-conserved regions and
helical exchange within the transmembrane segments, forms three channels
occupied by nascent cellulose polymers. Secretion steers the polymers
toward a common exit point, which could facilitate protofibril
formation. CesA's N-terminal domains assemble into a cytosolic stalk
that interacts with a microtubule-tethering protein and may thus be
involved in CesA localization.
|
| Accession Number: | O48946 |
|---|
| Protein Name: | Cellulose synthase A catalytic subunit 1 [UDP-forming] |
|---|
| Length: | 1081 |
|---|
| Molecular Weight: | 122237.00 |
|---|
| Species: | Arabidopsis thaliana (Mouse-ear cress) [3702] |
|---|
| Number of TMSs: | 8 |
|---|
| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
|---|
| Substrate |
(1->4)-beta-D-glucan |
|---|
1: MEASAGLVAG SYRRNELVRI RHESDGGTKP LKNMNGQICQ ICGDDVGLAE TGDVFVACNE
61: CAFPVCRPCY EYERKDGTQC CPQCKTRFRR HRGSPRVEGD EDEDDVDDIE NEFNYAQGAN
121: KARHQRHGEE FSSSSRHESQ PIPLLTHGHT VSGEIRTPDT QSVRTTSGPL GPSDRNAISS
181: PYIDPRQPVP VRIVDPSKDL NSYGLGNVDW KERVEGWKLK QEKNMLQMTG KYHEGKGGEI
241: EGTGSNGEEL QMADDTRLPM SRVVPIPSSR LTPYRVVIIL RLIILCFFLQ YRTTHPVKNA
301: YPLWLTSVIC EIWFAFSWLL DQFPKWYPIN RETYLDRLAI RYDRDGEPSQ LVPVDVFVST
361: VDPLKEPPLV TANTVLSILS VDYPVDKVAC YVSDDGSAML TFESLSETAE FAKKWVPFCK
421: KFNIEPRAPE FYFAQKIDYL KDKIQPSFVK ERRAMKREYE EFKVRINALV AKAQKIPEEG
481: WTMQDGTPWP GNNTRDHPGM IQVFLGHSGG LDTDGNELPR LIYVSREKRP GFQHHKKAGA
541: MNALIRVSAV LTNGAYLLNV DCDHYFNNSK AIKEAMCFMM DPAIGKKCCY VQFPQRFDGI
601: DLHDRYANRN IVFFDINMKG LDGIQGPVYV GTGCCFNRQA LYGYDPVLTE EDLEPNIIVK
661: SCCGSRKKGK SSKKYNYEKR RGINRSDSNA PLFNMEDIDE GFEGYDDERS ILMSQRSVEK
721: RFGQSPVFIA ATFMEQGGIP PTTNPATLLK EAIHVISCGY EDKTEWGKEI GWIYGSVTED
781: ILTGFKMHAR GWISIYCNPP RPAFKGSAPI NLSDRLNQVL RWALGSIEIL LSRHCPIWYG
841: YHGRLRLLER IAYINTIVYP ITSIPLIAYC ILPAFCLITD RFIIPEISNY ASIWFILLFI
901: SIAVTGILEL RWSGVSIEDW WRNEQFWVIG GTSAHLFAVF QGLLKVLAGI DTNFTVTSKA
961: TDEDGDFAEL YIFKWTALLI PPTTVLLVNL IGIVAGVSYA VNSGYQSWGP LFGKLFFALW
1021: VIAHLYPFLK GLLGRQNRTP TIVIVWSVLL ASIFSLLWVR INPFVDANPN ANNFNGKGGV
1081: F