1.I.1.1.5
The nuclear envelope consists of the outer and the inner nuclear membrane, the nuclear lamina and the nuclear pore complexes, which regulate nuclear import and export (Batsios et al. 2019). The major constituent of the nuclear lamina of Dictyostelium is the lamin NE81. It can form filaments like B-type lamins, and it interacts with Sun1, as well as with the LEM/HeH-family protein Src1. Sun1 and Src1 are nuclear envelope transmembrane proteins involved in the centrosome-nucleus connection and nuclear envelope stability at the nucleolar regions, respectively. In conjunction with a KASH-domain protein, Sun1 usually forms a so-called LINC complex. Two proteins with functions reminiscent of KASH-domain proteins at the outer nuclear membrane of Dictyostelium are known; interaptin which serves as an actin connector and the kinesin Kif9 which plays a role in the microtubule-centrosome connector, both of which lack the conserved KASH-domain. The link of the centrosome to the nuclear envelope is essential for the insertion of the centrosome into the nuclear envelope and appropriate spindle formation. Centrosome insertion is involved in permeabilization of the mitotic nucleus, which ensures access of tubulin dimers and spindle assembly factors (Batsios et al. 2019).
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| Accession Number: | O76329 |
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| Protein Name: | Interaptin |
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| Length: | 1738 |
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| Molecular Weight: | 204427.00 |
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| Species: | Dictyostelium discoideum (Slime mold) [44689] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Nucleus membrane1 / Single-pass type IV membrane protein2 / Cytoplasmic side3 |
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| Substrate |
protein, (ribonucleotide)n+m |
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1: MEHSTPLNEE IVHKKNDENW VIAQKKVFTN WCNIFLNQRS QKIEDLETDL YDGILLGSLL
61: EILSGKNVIL SKCKQLKTRL HYINNLNFSL KFIGDEGLRL VGVASEDITD GNLKLILGLV
121: WTLILRYQIQ SMQNSKSSQQ NLHSSTKPSE LMLNWVKSQI SDYGHHIKDL TTSFQNGLLF
181: CALVHKLVPE KLDYKSLSES DSLGNLTLAF EVANKELGIP SILDPHDIIT TPDELSILTY
241: ISLFPKVYQQ TLEPLNNNNN ISPSLSSSSS SLLNTPNKRN SIQLSKSTSF EQQNQQQQQQ
301: NLLSPNSYRN SISFSKSPSF EGSQSTGSSR SISPISSPIK NSTTGNSNLS KSTSFEKIEA
361: SNTTNNNTII IAEESRVIEK IVEKIIEVEK IVEVEKIVEV EKIVEVEKIV EVEKIVKVDD
421: IEKLTNLQDQ LTEQQQQYQE KSLKLVNLEL ELQEKSNQLV DKSNQLSTMQ ATNSELMEKI
481: GGLMNDLTDI PTQDIKEKDE IIANLKIESE KNLKCFQDDF NALQSRYSLT IEQTSQLQDR
541: IKQLINELQE RDDKFIEFTN SSNQSLADNQ RVIDQLTNEK QSITLQLQDQ QDIKEKEFQF
601: EKQQLLSQID SITTNIQEYQ DKFNNLQQEF NTQQTLNQQE THRLTQQLYQ INTDYNEKQT
661: QLQSEIKDNQ TINEQLNKQL SEKDKEIEKL SNQQEQQQDE KINNLLLEIK EKDCLIERIN
721: QQLLENIDLN SKYQQLLLEF ENFKLNSSKE KENQLNELQS KQDERFNQLN DEKLEKEKQL
781: QSIEDEFNQY KQQQLSSNSN IDQQLQSTII ELSELKEQKE LNDSKLIEKE KQLQQLQQEF
841: DQLNEKNQKD HQDQLELLEK QLKQLQQEYD QLNETNQSIE NQLNQQNLIN KENLNEKEQE
901: LLKLQNQLNQ QIEKIQFDQQ EFSKQNSINI ELVNEKNEKL IQLQQDYDQL KQQNRSNDEK
961: DENDLIEKEN QLKSIQNELN QLIEKNESDH KEQQLKQQSI ENDLIEKENQ IQQLQSQLNE
1021: QRQQQSNQLS EKDQQLNQLI EKNQFDQKEQ QLKQQSIEND LFEKENQIQQ LQSQLNEQRQ
1081: QQSNQLSEKD QQLNQLIEKN ESDQKEQQLK QQSIENDLIE KENQIQQLQL QLNEQRQLQS
1141: EVSIDNDKIL ELEKQLKQCQ SDLLKLNDEK QQQDKQLQDK QIEFDQLQLT FNQFKNDKDS
1201: QFIQLQDDQK QQLQSIQQDL NQLKQENQEK EKQLSEKDEK LQSIQFENQE KEKQLSEKDE
1261: KLQSIQQNLN QLNDENQEKV KQFSEKDEKL QSIQQDLNQL KQENQEKEKQ LSEKDEKLQS
1321: IQQDLNQLND DQIKKNEKLK EKEEQLLKLQ QDFNDQQSQQ LKQLEEKLSE KENQLQQLKQ
1381: ENEINQLNQQ QQSNEIIQQL KDQLLKQQQQ EQQENNNEKE IERLIQEIEQ LKQQQEIDQS
1441: ELSNKEIKIQ TTQQEFDQLS HNRSKDQLHL QQLQQELDQL KQSFDDQDHQ FKKVIDERYN
1501: LQLQLEQSTL SNNQLDQLLK EKLKPLELDS NEKQKTIDDL LSNISNLQIS LQNDKDLISE
1561: RNNSIKTLES RITQQLSLLD EKDNLIKDLQ QQKQQQQQPP TASSSPSSSP SLLSSTPTPK
1621: PQRPNQIEID RLVNEIVNRN QDLIRKNKTK FYKLENGDYI VNSIIYRLSL DDDNDSDLIA
1681: QEYENGNSTT FEKSLRIFPS KNTRPIFDWR ALFFIGAAVL AISTLFSSSR PIKYEKPT