TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.9.1.9
The cation-selective pentameric nicotinic acetylcholine receptor, nAChR, with α (461 aas; P02710), β (493 aas; P02712), γ (506 aas; P02714) and δ (522 aas; P02718) subunits.  The transmembrane domain of the uncoupled nAChR adopts a conformation distinct from that of the resting or desensitized state (Sun et al. 2016).  Studies with this receptor have been reviewed (Unwin 2013).  Many small molecules interact with nAChRs including d-tubocurarine, snake venom protein α-bungarotoxin (α-Bgt), and α-conotoxins, neurotoxic peptides from Conus snails. Various more recently discovered compounds of different structural classes also interact with nAChRs including the low-molecular weight alkaloids, pibocin, varacin and makaluvamines C and G. 6-Bromohypaphorine from the mollusk Hermissenda crassicornis does not bind to Torpedo nAChR but behaves as an agonist on human α7 nAChR (Kudryavtsev et al. 2015). Dimethylaniline mimics the low potency and non-competitive actions of lidocaine on nAChRs, as opposed to the high potency and voltage-dependent block by lidocaine (Alberola-Die et al. 2016).  Cholesterol is a potent modulator of the Torpedo nAChR (Baenziger et al. 2017). Cholesterol may play a mechanical role by conferring local rigidity to the membrane so that there is productive coupling between the extracellular and membrane domains, leading to opening of the channel (Unwin 2017). 11beta-(p-azidotetrafluorobenzoyloxy)allopregnanolone (F4N3Bzoxy-AP), a general anesthetic, a photoreactive allopregnanolone analog and a potent GABAAR PAM,was used to characterize steroid binding sites in the Torpedo nAChR in its native membrane environment (Yu et al. 2019). The steroid-binding site in the nAChR ion channel was identified, and additional steroid-binding sites could also be occupied by other lipophilic nAChR antagonists. Structural features of the αM4 TMS determine how lipid dependent changes in alphaM4 structure may ultimately modify nAChR function (Thompson et al. 2020).

Accession Number:P02712
Protein Name:Acetylcholine receptor subunit beta
Length:493
Molecular Weight:56156.00
Species:Tetronarce californica (Pacific electric ray) [7787]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell junction1 / Multi-pass membrane protein2
Substrate ions

Cross database links:

Structure:
1oed   4aq5   6UWZ     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MENVRRMALG LVVMMALALS GVGASVMEDT LLSVLFETYN PKVRPAQTVG DKVTVRVGLT 
61:	LTNLLILNEK IEEMTTNVFL NLAWTDYRLQ WDPAAYEGIK DLRIPSSDVW QPDIVLMNNN 
121:	DGSFEITLHV NVLVQHTGAV SWQPSAIYRS SCTIKVMYFP FDWQNCTMVF KSYTYDTSEV 
181:	TLQHALDAKG EREVKEIVIN KDAFTENGQW SIEHKPSRKN WRSDDPSYED VTFYLIIQRK 
241:	PLFYIVYTII PCILISILAI LVFYLPPDAG EKMSLSISAL LAVTVFLLLL ADKVPETSLS 
301:	VPIIIRYLMF IMILVAFSVI LSVVVLNLHH RSPNTHTMPN WIRQIFIETL PPFLWIQRPV 
361:	TTPSPDSKPT IISRANDEYF IRKPAGDFVC PVDNARVAVQ PERLFSEMKW HLNGLTQPVT 
421:	LPQDLKEAVE AIKYIAEQLE SASEFDDLKK DWQYVAMVAD RLFLYVFFVI CSIGTFSIFL 
481:	DASHNVPPDN PFA