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3.A.1.7.1
Phosphate porter, PhoSPstABC.  Serves as both a transporter and a sensor for transcriptional activation of the pho regulon in the presence of low external phosphate.  The unphosphorylated EIIANtr protein of the PTS (TC# 4.A) activates PhoR, the senor kinase that phosphorylates the response regulator, PhoB, that activates the pho regulon (Lüttmann et al. 2012).

Accession Number:P07654
Protein Name:PstA aka PhoT aka B3726
Length:296
Molecular Weight:32322.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate phosphate(3-)

Cross database links:

RefSeq: AP_004061.1    NP_418182.1   
Entrez Gene ID: 948239   
Pfam: PF00528   
BioCyc: EcoCyc:PSTA-MONOMER    ECOL168927:B3726-MONOMER   
KEGG: ecj:JW3704    eco:b3726   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005315 F:inorganic phosphate transmembrane transport...
GO:0006817 P:phosphate transport

References (8)

[1] “Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli.”  Amemura M.et.al.   2993631
[2] “Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.”  Surin B.P.et.al.   3881386
[3] “DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.”  Burland V.D.et.al.   7686882
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices.”  Webb D.C.et.al.   1447208
[7] “Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis.”  Drew D.et.al.   11867724
[8] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MAMVEMQTTA ALAESRRKMQ ARRRLKNRIA LTLSMATMAF GLFWLIWILM STITRGIDGM 
61:	SLALFTEMTP PPNTEGGGLA NALAGSGLLI LWATVFGTPL GIMAGIYLAE YGRKSWLAEV 
121:	IRFINDILLS APSIVVGLFV YTIVVAQMEH FSGWAGVIAL ALLQVPIVIR TTENMLKLVP 
181:	YSLREAAYAL GTPKWKMISA ITLKASVSGI MTGILLAIAR IAGETAPLLF TALSNQFWST 
241:	DMMQPIANLP VTIFKFAMSP FAEWQQLAWA GVLIITLCVL LLNILARVVF AKNKHG