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2.C.1.2.1
The TolA energy-transducing system. The H+-channel-forming TolQ/R proteins are listed under TC# 1.A.30.2.2.  Proteins of the complex listed here are TolA, TolB, YbgF and Pal.  They play a role in outer membrane stabilization and uptake of colicins, but not energizaton of outer membrane receptors (OMRs: TC# 1.B.14).  May function in phospholipid transport (SS Chng, personal communication).  Cells lacking the Tol-Pal complex exhibit defects in lipid asymmetry and accumulate excess phospholipids (PLs) in the OM. This imbalance in OM lipids is due to defective retrograde PL transport in the absence of a functional Tol-Pal complex. Thus, cells ensure the assembly of a stable OM by maintaining an excess flux of PLs to the OM only to return the surplus to the inner membrane via transport mediated by the Tol-Pal complex (Shrivastava et al. 2017).  The specific function of the Tol-Pal complex may be to transport lipids from the outer leaflet of the outer membrane to the inner leaflet of the outer member. The Tol-Pal complex is essential to maintain polar localization of the MCP chemotaxis receptor complex (Santos et al. 2014). The Tol-Pal complex, energized by TolQRA, and using the outer membrane proteins, BtuB and OmpF as receptors, is responsible for the uptake of colicin ColE9 and other bacteriocins; in this process, the complex in the outer membrane bridges and immobilizes the complex components in the inner membrane (Rassam et al. 2018).

Accession Number:P0A855
Protein Name:Protein TolB
Length:430
Molecular Weight:45956.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Periplasm1
Substrate phospholipid

Cross database links:

DIP: DIP-48262N
Structure:
1C5K   1CRZ   2HQS   2IVZ   2W8B   3IAX     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA PEDIGGIVAA 
61:	DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV VGQVTPNPDG SYNVAYQLVD 
121:	TGGAPGTVLA QNSYKVNKQW LRYAGHTASD EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY 
181:	ELRVSDYDGY NQFVVHRSPQ PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA 
241:	SFPRHNGAPA FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ 
301:	NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS SNGGQQHIAK 
361:	QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV LNLVSTDGRF KARLPATDGQ 
421:	VKFPAWSPYL