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2.C.1.2.1
The TolA energy-transducing system. The H+-channel-forming TolQ/R proteins are listed under TC# 1.A.30.2.2.  Proteins of the complex listed here are TolA, TolB, YbgF and Pal.  They play a role in outer membrane stabilization and uptake of colicins, but not energizaton of outer membrane receptors (OMRs: TC# 1.B.14).  May function in phospholipid transport (SS Chng, personal communication).  Cells lacking the Tol-Pal complex exhibit defects in lipid asymmetry and accumulate excess phospholipids (PLs) in the OM. This imbalance in OM lipids is due to defective retrograde PL transport in the absence of a functional Tol-Pal complex. Thus, cells ensure the assembly of a stable OM by maintaining an excess flux of PLs to the OM only to return the surplus to the inner membrane via transport mediated by the Tol-Pal complex (Shrivastava et al. 2017).  The specific function of the Tol-Pal complex may be to transport lipids from the outer leaflet of the outer membrane to the inner leaflet of the outer member. The Tol-Pal complex is essential to maintain polar localization of the MCP chemotaxis receptor complex (Santos et al. 2014). The Tol-Pal complex, energized by TolQRA, and using the outer membrane proteins, BtuB and OmpF as receptors, is responsible for the uptake of colicin ColE9 and other bacteriocins; in this process, the complex in the outer membrane bridges and immobilizes the complex components in the inner membrane (Rassam et al. 2018).

Accession Number:P19934
Protein Name:TolA aka CIM aka EXCC aka LKY aka B0739
Length:421
Molecular Weight:43157.00
Species:Escherichia coli [83333]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Single-pass type II membrane protein2
Substrate phospholipid

Cross database links:

DIP: DIP-11005N
RefSeq: AP_001377.1    NP_415267.1   
Entrez Gene ID: 946625   
Pfam: PF06519   
BioCyc: EcoCyc:EG11007-MONOMER    ECOL168927:B0739-MONOMER   
KEGG: ecj:JW0729    eco:b0739   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005515 F:protein binding
GO:0043213 P:bacteriocin transport
GO:0017038 P:protein import

References (7)

[1] “Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli.”  Levengood S.K.et.al.   2687247
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “TolA: a membrane protein involved in colicin uptake contains an extended helical region.”  Levengood S.K.et.al.   2068069
[6] “TolA central domain interacts with Escherichia coli porins.”  Derouiche R.et.al.   8978668
[7] “Filamentous phage infection: crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA.”  Lubkowski J.et.al.   10404600
Structure:
1S62   1TOL   3QDR   3QDP     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSKATEQNDK LKRAIIISAV LHVILFAALI WSSFDENIEA SAGGGGGSSI DAVMVDSGAV 
61:	VEQYKRMQSQ ESSAKRSDEQ RKMKEQQAAE ELREKQAAEQ ERLKQLEKER LAAQEQKKQA 
121:	EEAAKQAELK QKQAEEAAAK AAADAKAKAE ADAKAAEEAA KKAAADAKKK AEAEAAKAAA 
181:	EAQKKAEAAA AALKKKAEAA EAAAAEARKK AATEAAEKAK AEAEKKAAAE KAAADKKAAA 
241:	EKAAADKKAA EKAAAEKAAA DKKAAAEKAA ADKKAAAAKA AAEKAAAAKA AAEADDIFGE 
301:	LSSGKNAPKT GGGAKGNNAS PAGSGNTKNN GASGADINNY AGQIKSAIES KFYDASSYAG 
361:	KTCTLRIKLA PDGMLLDIKP EGGDPALCQA ALAAAKLAKI PKPPSQAVYE VFKNAPLDFK 
421:	P