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3.A.1.11.2
Putrescine porter (Igarashi and Kashiwagi 1996).

Accession Number:P31133
Protein Name:PotF aka B0854
Length:370
Molecular Weight:40840.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Periplasm1
Substrate putrescine

Cross database links:

DIP: DIP-10531N
RefSeq: AP_001485.1    NP_415375.1   
Entrez Gene ID: 945480   
Pfam: PF01547   
BioCyc: EcoCyc:POTF-MONOMER    ECOL168927:B0854-MONOMER   
KEGG: ecj:JW0838    eco:b0854   

Gene Ontology

GO:0030288 C:outer membrane-bounded periplasmic space
GO:0015594 F:putrescine-importing ATPase activity
GO:0015847 P:putrescine transport

References (7)

[1] “Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.”  Pistocchi R.et.al.   8416922
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link A.J.et.al.   9298646
[6] “Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli.”  Vassylyev D.G.et.al.   9761835
[7] “Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity.”  Vassylyev D.G.et.al.   9651355
Structure:
1A99   4JDF     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTALNKKWLS GLVAGALMAV SVGTLAAEQK TLHIYNWSDY IAPDTVANFE KETGIKVVYD 
61:	VFDSNEVLEG KLMAGSTGFD LVVPSASFLE RQLTAGVFQP LDKSKLPEWK NLDPELLKLV 
121:	AKHDPDNKFA MPYMWATTGI GYNVDKVKAV LGENAPVDSW DLILKPENLE KLKSCGVSFL 
181:	DAPEEVFATV LNYLGKDPNS TKADDYTGPA TDLLLKLRPN IRYFHSSQYI NDLANGDICV 
241:	AIGWAGDVWQ ASNRAKEAKN GVNVSFSIPK EGAMAFFDVF AMPADAKNKD EAYQFLNYLL 
301:	RPDVVAHISD HVFYANANKA ATPLVSAEVR ENPGIYPPAD VRAKLFTLKV QDPKIDRVRT 
361:	RAWTKVKSGK