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3.A.1.105.1
Daunorubicin, doxorubicin etc. (multidrug resistance) exporter, DrrAB.  DrrB binds drugs with variable affinities and contains multiple drug binding sites. The two asymmetric nucleotide binding sites in DrrA have strikingly different binding affinities. Long-range conformational changes occur between DrrA and DrrB. The transduction pathway from the nucleotide-binding DrrA subunit to the substrate binding DrrB subunit includes the Q-loop and CREEM motifs in DrrA and the EAA-like motif in DrrB (Rahman and Kaur 2018).

Accession Number:P32011
Protein Name:DrrB
Length:283
Molecular Weight:30614.00
Species:Streptomyces peucetius [1950]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate daunorubicin, doxorubicin

Cross database links:

Pfam: PF01061   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0046677 P:response to antibiotic
GO:0006810 P:transport

References (3)

[1] “A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin.”  Guilfoile P.G.et.al.   1924314
[2] “Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein.”  Kaur P.et.al.   9006006
[3] “Membrane topology of the DrrB protein of the doxorubicin transporter of Streptomyces peucetius.”  Gandlur S.M.et.al.   15090538

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTTSPGTVES TTPVSGQLRT VLSAGERPAR ATAVSATLTH LWRAMMAFKH FPVQLIDIVL 
61:	MPLIFLLMFT YLFGGAFADS TEEYLQFYLP GVTVQAVVMM TVYTGTSLNT DIHKGVFDRF 
121:	RTLPFWQPAT LAGSLLGDVL RYVVALATTV SLGLLLGFRA DGGFLGVVGA MLVLIVFGFS 
181:	VSWIFAALGV VASEPERVSG TSMIVLYPLL FMSNIFVMPE TMPGWMQAIV DANPMSHAAT 
241:	ASRELMHGTA GFWDVGLVLC VSAGLVAVFA PLTMRLYRNK NAH