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9.A.8.1.1
Ferrous iron uptake system, FeoAB.  FeoB is the main transporter while FeoC is a transcriptional regulator.  FeoA has a Src-Homology (SH3) domain (a β-barrel with two overlying α-helices) plus two extra α-helices not usually found in SH3 domains.  FeoA interacts with the cytoplasmic domain of the conserved core portion of the FeoB transmembrane region, but may not function as a GTPase activator as previously proposed (Lau et al. 2012).   FeoB transports Fe(II) from the periplasm to the cytoplasm, but the C-terminal domain can also bind Mn2+ and Zn2+ as well (Orzel et al. 2023).

Accession Number:P33650
Protein Name:FeoB aka B3409
Length:773
Molecular Weight:84474.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate iron(2+), zinc(2+), manganese(2+)

Cross database links:

DIP: DIP-9591N
RefSeq: AP_004381.1    NP_417868.1   
Entrez Gene ID: 947919   
Pfam: PF07664    PF02421    PF07670   
BioCyc: EcoCyc:FEOB-MONOMER    ECOL168927:B3409-MONOMER   
KEGG: ecj:JW3372    eco:b3409   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015093 F:ferrous iron transmembrane transporter acti...
GO:0005525 F:GTP binding
GO:0001882 F:nucleoside binding
GO:0015684 P:ferrous iron transport

References (6)

[1] “Characterization of the ferrous iron uptake system of Escherichia coli.”  Kammler M.et.al.   8407793
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations.”  Lenski R.E.et.al.   12664169
[5] “The membrane protein FeoB contains an intramolecular G protein essential for Fe(II) uptake in bacteria.”  Marlovits T.C.et.al.   12446835
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
3HYR   3HYT   3I8S   3I8X   3I92   4Q00   4Q5I   4R98     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MKKLTIGLIG NPNSGKTTLF NQLTGSRQRV GNWAGVTVER KEGQFSTTDH QVTLVDLPGT 
61:	YSLTTISSQT SLDEQIACHY ILSGDADLLI NVVDASNLER NLYLTLQLLE LGIPCIVALN 
121:	MLDIAEKQNI RIEIDALSAR LGCPVIPLVS TRGRGIEALK LAIDRYKANE NVELVHYAQP 
181:	LLNEADSLAK VMPSDIPLKQ RRWLGLQMLE GDIYSRAYAG EASQHLDAAL ARLRNEMDDP 
241:	ALHIADARYQ CIAAICDVVS NTLTAEPSRF TTAVDKIVLN RFLGLPIFLF VMYLMFLLAI 
301:	NIGGALQPLF DVGSVALFVH GIQWIGYTLH FPDWLTIFLA QGLGGGINTV LPLVPQIGMM 
361:	YLFLSFLEDS GYMARAAFVM DRLMQALGLP GKSFVPLIVG FGCNVPSVMG ARTLDAPRER 
421:	LMTIMMAPFM SCGARLAIFA VFAAAFFGQN GALAVFSLYM LGIVMAVLTG LMLKYTIMRG 
481:	EATPFVMELP VYHVPHVKSL IIQTWQRLKG FVLRAGKVII IVSIFLSAFN SFSLSGKIVD 
541:	NINDSALASV SRVITPVFKP IGVHEDNWQA TVGLFTGAMA KEVVVGTLNT LYTAENIQDE 
601:	EFNPAEFNLG EELFSAIDET WQSLKDTFSL SVLMNPIEAS KGDGEMGTGA MGVMDQKFGS 
661:	AAAAYSYLIF VLLYVPCISV MGAIARESSR GWMGFSILWG LNIAYSLATL FYQVASYSQH 
721:	PTYSLVCILA VILFNIVVIG LLRRARSRVD IELLATRKSV SSCCAASTTG DCH