1.I.1.1.5
The nuclear envelope consists of the outer and the inner nuclear membrane, the nuclear lamina and the nuclear pore complexes, which regulate nuclear import and export (Batsios et al. 2019). The major constituent of the nuclear lamina of Dictyostelium is the lamin NE81. It can form filaments like B-type lamins, and it interacts with Sun1, as well as with the LEM/HeH-family protein Src1. Sun1 and Src1 are nuclear envelope transmembrane proteins involved in the centrosome-nucleus connection and nuclear envelope stability at the nucleolar regions, respectively. In conjunction with a KASH-domain protein, Sun1 usually forms a so-called LINC complex. Two proteins with functions reminiscent of KASH-domain proteins at the outer nuclear membrane of Dictyostelium are known; interaptin which serves as an actin connector and the kinesin Kif9 which plays a role in the microtubule-centrosome connector, both of which lack the conserved KASH-domain. The link of the centrosome to the nuclear envelope is essential for the insertion of the centrosome into the nuclear envelope and appropriate spindle formation. Centrosome insertion is involved in permeabilization of the mitotic nucleus, which ensures access of tubulin dimers and spindle assembly factors (Batsios et al. 2019).
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| Accession Number: | P34036 |
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| Protein Name: | Dynein heavy chain, cytoplasmic |
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| Length: | 4730 |
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| Molecular Weight: | 539613.00 |
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| Species: | Dictyostelium discoideum (Slime mold) [44689] |
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| Location1 / Topology2 / Orientation3: |
Cytoplasm1 |
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| Substrate |
protein, (ribonucleotide)n+m |
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1: MEDQQINVDS PTSGTNTPPV VTPTTSVISE QTLEETVKYL CKICPTLLDG DQSVFQNNLS
61: NQIPPENMNK LKKFISDSKI PVLLIQKTNP TINSSNQTST TTSSSSSSDD NTLTSSQQQS
121: KESFNFEIEV KFGGENKSTL AIVKRIPESI VEYSSNKSIA SQLQVLNLGD GSPMDTLHNY
181: IHNSVAPFVR SYILSASKDD ATTPSGGSAD KSITSQNLDK EMKQSIGAVN QKIAELEISL
241: YNCKQQVQIP EVTLAINPEI KSISKRLKET TGRTIKPDDL GDKASSPEFL NLLQAGTTTW
301: AKNIQNVTKH NLIENLPSDV STSQEINFWI ELETSLQNID QQLKSPEVEV TLATLRQAKR
361: FIASAPFETD TIGVRKAMDK VQSYKTLFKD FPITPLLTAT DLDSISSSVA AIFSHLKKTK
421: NPYYPIPRYL SFLEAIGRDM CNKVYQILRQ KNLMNIDYND FEHLNRSVRA LFTLWDDQFG
481: AFRDILRDLA KKRGNERIPL IVNIDNRIQL VIIKIGKFRK QHEDLKNVVS NVLPGSQLGG
541: GVVQTNTSNP TSPQKQEINA IEEINQAYLE FKEIDVLQLS KEGEEIWDAV VKRYNSRTDR
601: VETYITVKLR DRLATAKNAN EMFRVYQKFK DLLKRPKIRG ATHEYESQLI ERVKEDIRVL
661: HDKFKMQYNN SEAYYMSQLR DLPPVSGAII WARQIERQLD TYMKRVANVL GDSWESDAEG
721: QKLKSESDQF RHKLNTDHIF SKWADETEKR SFDISGRILT IVKRGNKLAL DINFDSHIIM
781: LFKEVRNLQW LGFRVPLKIS FISQGAKQVY PFAVSLKETL RTYAQTSGKV TPEFSTLVAS
841: YKRDVQANIT EGFRLKWETI PKVDPYVRKL STSINNFRDK VDDLIVKYSE IKKQLDGLKS
901: CPFKSESFNE IIANIQKVVD ELNLANYSNL PQWVTQLDAQ VESMLIERLI DAINSWVQLI
961: EGKEDQKDSQ STSGSSNKGG KLNRMNYSIR NKSDEENSSD LTQPQQSQQQ QQTISIKPKL
1021: EKTIHEIVIR NQILSLSPPL EVARVNWIDQ LHSWLNICCD LPRIQSSRYD ESAMVHRGGV
1081: DSKKQSTFRD MLPKLPQGSL ESAYSAITNK LEQVQQYVSI WLQYQSLWDM DSSFVYSKLG
1141: DDLNKWQLLL NQIKKSRSTF DNSSTEKQFG PLTIDYTQVQ ASVNNKYDYW HKDILGHFGS
1201: KLAEKMNQFY ETISSSRQEL EKLSVETVST EEAVHFIIQI QDMKKKLSSW EADLRYYRTG
1261: QDLLQRQRFS FPNDWLDCER VEGEWSAFNE ILNRKNATIS EAIPQLQAKI LQESKSINDR
1321: IKDFIDEWTA NKPLQGSIKH STALETLKIF EGRLIRLREE SDRLSKAKQA LDLTDTTGSS
1381: SSDQDRLVPV EEEIQDLKAV WVELSNTWQE IDSLKETAWS AIIPRKVRKS LEDTLQKLKN
1441: LPNRIRQYSA FDHAQNLIKI YLKGNAIITD LHSEAIKDRH WKILKKRLNT NWIITELTLG
1501: SIWDSDLARN ENIYREVITA AQGEIALEEF LKGVREFWTT LELDLVNYQR KCKLVRGWDD
1561: LFNKLAEHLN SISAMKMSPY YKVFEEEANH WDDRLNKVRS LLDVWIDVQR RWVYLEGIFS
1621: GSGDINQLLP AESTRFKSIN SEFIAILKKV SGAPLILEVL AIERIQQTME RLSDLLGKVQ
1681: KALGEYLERQ RSAFARFYFV GDEDLLEIIG NSKDIIKIQK HFRKMFAGLA NLTLDDEKTT
1741: IIGMSSAEGE TVTFKKPISI ANGPKIHEWL TMVESEMKST LATLLSESLQ HFNQVDVNDH
1801: SKYSEWVDNY PTQLVLLTSQ IVWSTQVDQA LGGGTLQQSK IQEQLQSIEQ TTQMILNNLA
1861: DSVLQDLSAQ KRKKFEHLIT ELVHQRDVVR QLQKCKNLTG NKDFDWLYHM RYYYDATQEN
1921: VLHKLVIHMA NATFYYGFEY LGIGERLVQT PLTDRCYLTL TQALESRMGG NPFGPAGTGK
1981: TETVKALGSQ LGRFVLVFCC DEGFDLQAMS RIFVGLCQCG AWGCFDEFNR LEERILSAVS
2041: QQIQTIQVAL KENSKEVELL GGKNISLHQD MGIFVTMNPG YAGRSNLPDN LKKLFRSMAM
2101: IKPDREMIAQ VMLYSQGFKT AEVLAGKIVP LFKLCQEQLS AQSHYDFGLR ALKSVLVSAG
2161: GIKRKCQPPQ LPPITDAESK TKADQIYCQY EIGVLLNSIN DTMIPKLVAD DIPLIQSLLL
2221: DVFPGSQLQP IQMDQLRKKI QEIAKQRHLV TKQEWVEKIL QLHQILNINH GVMMVGPSGG
2281: GKTTSWEVYL EAIEQVDNIK SEAHVMDPKA ITKDQLFGSL DLTTREWTDG LFTATLRRII
2341: DNVRGESTKR HWIIFDGDVD PEWVENLNSL LDDNKLLTLP NGERLALPNN VRVMFEVQDL
2401: KYATLATISR CGMVWFSEEI LTTQMIFQNY LDTLSNEPFD PQEKEQQKRN ENAQLQQQQQ
2461: TTITSPILTS PPTTSSSSRS TTSTTSMIPA GLKVQKECAA IISQYFEPGG LVHKVLEDAG
2521: QRPHIMDFTR LRVLNSFFSL MNRSIVNVIE YNQLHSDFPM SPENQSNYIT NRLLYSLMWG
2581: LGGSMGLVER ENFSKFIQTI AITPVPANTI PLLDYSVSID DANWSLWKNK VPSVEVETHK
2641: VASPDVVIPT VDTTRHVDVL HAWLSEHRPL ILCGPPGSGK TMTLTSTLRA FPDFEVVSLN
2701: FSSATTPELL LKTFDHHCEY KRTPSGETVL RPTQLGKWLV VFCDEINLPS TDKYGTQRVI
2761: TFIRQMVEKG GFWRTSDHTW IKLDKIQFVG ACNPPTDAGR VQLTHRFLRH APILLVDFPS
2821: TSSLTQIYGT FNRALMKLLP NLRSFADNLT DAMVEFYSES QKRFTPDIQA HYIYSPRELS
2881: RWDRALLEAI QTMDGCTLEG LVRLWAHEAL RLFQDRLVET EEKEWTDKKI DEVALKHFPS
2941: VNLDALKRPI LYSNWLTKDY QPVNRSDLRE YVKARLKVFY EEELDVPLVL FNEVLDHILR
3001: IDRVFRQPQG HALLIGVSGG GKSVLSRFVA WMNGLSIYTI KVNNNYKSSD FDDDLRMLLK
3061: RAGCKEEKIC FIFDESNVLE SSFLERMNTL LAGGEVPGLF EGEEFTALMH ACKETAQRNG
3121: LILDSEEELY KYFTSQVRRN LHVVFTMNPA SPDFHNRSAT SPALFNRCVL DWFGEWSPEA
3181: LFQVGSEFTR NLDLENPQYI APPVFIQEAE IMGNNLMAIP PSHRDAVVSS LVYIHQTIGE
3241: ANIRLLKRQG RQNYVTPRHY LDFINQVVLL INEKRDQLEE EQLHLNIGLK KLRDTEAQVK
3301: DLQVSLAQKN RELDVKNEQA NQKLKQMVQD QQAAEIKQKD ARELQVQLDV RNKEIAVQKV
3361: KAYADLEKAE PAIIEAQEAV STIKKKHLDE IKSLPKPPTP VKLAMEAVCL MLGGKKLEWA
3421: DIRKKIMEPN FITSIINYDT KKMMTPKIRE AITKGYLEDP GFDYETVNRA SKACGPLVKW
3481: ATAQTYYSEI LDRIKPLREE VEQLENAANE LKLKQDEIVA TITALEKSIA TYKEEYATLI
3541: RETEQIKTES SKVKNKVDRS IALLDNLNSE RGRWEQQSEN FNTQMSTVVG DVVLASAFLA
3601: YIGFFDQNFR TDLMRKWMIR LDSVGIKFKS DLSVPSFLSK PEERLNWHAN SLPSDELCIE
3661: NAIMLKRFNR YPLVIDPSGQ AMEFLMNQYA DKKITKTSFL DSSFMKNLES ALRFGCPLLV
3721: QDVENIDPVL NPVLNKEIRK KGGRILIRLG DQDVDFSPSF MIFLFTRDPT AHFTPDLCSR
3781: VTFVNFTVTP SSLQSQCLHE ALKTERPDTH KKRSDLLKIQ GEFQVKLRIL EKSLLNALSQ
3841: ASGNILDDDS VISTLETLKK ETTEIALKVE ETETVMQEIS EVSALYNPMA LSCSRVYFAM
3901: EELSQFHLYQ FSLRAFLDIF YNLLNNNPNL VDKKDPNERL VYLSKDIFSM TFNRVTRTLL
3961: NDDKLTFALQ LTIISVKGTS NEIEESEWDF LLKGGDNLTS IKETIPQLDS LLSTTQQKWL
4021: ICLRQQVPSF SKLVDHIQQN SSDWKQFFGK DQVGEPIIPE SWIVAQAQLS NQQSTIVSNF
4081: RKILLMKAFH SDRVLQYSHS FVCSVFGEDF LNTQELDMAN IVEKEVKSSS PLLLCSVPGY
4141: DASSKVDDLA LQLHKQYKSF AIGSPEGFEL AEKSIYAAAK SGTWVLLKNI HLAPQWLVQL
4201: EKKLHSLSPH PSFRLFMTSE IHPALPANLL RMSNVFSYEN PPGVKANLLH TFIGIPATRM
4261: DKQPAERSRI YFLLAWFHAI IQERLRYIPL GWTKFFEFND ADLRGALDSI DYWVDLYSKG
4321: RSNIDPDKIP WIAVRTILGS TIYGGRIDNE FDMRLLYSFL EQLFTPSAFN PDFPLVPSIG
4381: LSVPEGTTRA HFMKWIEALP EISTPIWLGL PENAESLLLS NKARKMINDL QKMQSSEEDG
4441: EDDQVSGSSK KESSSSSSED KGKAKLRATI TEWTKLLPKP LKQLKRTTQN IKDPLFRCFE
4501: REISTGGKLV KKITNDLANL LELISGNIKS TNYLRSLTTS ISKGIVPKEW KWYSVPETIS
4561: LSVWISDFSK RMQQLSEISE SSDYSSIQVW LGGLLNPEAY ITATRQSASQ LNGWSLENLR
4621: LHASSLGKIS SEGGASFNVK GMALEGAVWN NDQLTPTDIL STPISIATLT WKDKDDPIFN
4681: NSSSKLSVPV YLNETRSELL FSIDLPYDQS TSKQNWYQRS VSISSWKSDI