4.D.3.1.6
Core cellulose synthase complex, BcsA/BcsB (Morgan et al. 2013; Omadjela et al. 2013). BcsA (YhjO) is an 10 - 12 TMS protein with 4 N-terminal TMSs, the glycosyl transferase domain and 4 C-terminal TMSs. It is believed to both synthesize the glycosyl linkages and transport the polysaccharide across the membrane (Römling and Galperin 2015). BcsB (YhjN) is a cellulose synthase regulator, a cyclic diGMP binding protein, with two TMSs at the N- and C-termini and the bulk of the protein in the periplasm. BcsC (TC# 1.B.55.3.1) is the outer membrane porin for the export of cellulose with an N-terminal α-TMS, a large N-terminal hydrophylic domain, and a C-terminal β-barrel domain. Two other proteins, an endoglucanase, BscZ or YhjM, and a diguanylate cyclase, YedQ (TC# 9.B.34.1.4) may play roles in cellulose biosynthesis (Imai et al. 2014). Cellulose is a component of the bacterial extracellular matrix (Zogaj et al. 2001) and plays roles in biofilm formation and cell adhesion (Hu et al. 2015).
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| Accession Number: | P37652 |
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| Protein Name: | Cyclic di-GMP-binding protein |
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| Length: | 779 |
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| Molecular Weight: | 86024.00 |
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| Species: | Escherichia coli (strain K12) [83333] |
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| Number of TMSs: | 2 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Single-pass type I membrane protein2 |
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| Substrate |
(1->4)-beta-D-glucan |
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1: MKRKLFWICA VAMGMSAFPS FMTQATPATQ PLINAEPAVA AQTEQNPQVG QVMPGVQGAD
61: APVVAQNGPS RDVKLTFAQI APPPGSMVLR GINPNGSIEF GMRSDEVVTK AMLNLEYTPS
121: PSLLPVQSQL KVYLNDELMG VLPVTKEQLG KKTLAQMPIN PLFISDFNRV RLEFVGHYQD
181: VCEKPASTTL WLDVGRSSGL DLTYQTLNVK NDLSHFPVPF FDPSDNRTNT LPMVFAGAPD
241: VGLQQASAIV ASWFGSRSGW RGQNFPVLYN QLPDRNAIVF ATNDKRPDFL RDHPAVKAPV
301: IEMINHPQNP YVKLLVVFGR DDKDLLQAAK GIAQGNILFR GESVVVNEVK PLLPRKPYDA
361: PNWVRTDRPV TFGELKTYEE QLQSSGLEPA AINVSLNLPP DLYLMRSTGI DMDINYRYTM
421: PPVKDSSRMD ISLNNQFLQS FNLSSKQEAN RLLLRIPVLQ GLLDGKTDVS IPALKLGATN
481: QLRFDFEYMN PMPGGSVDNC ITFQPVQNHV VIGDDSTIDF SKYYHFIPMP DLRAFANAGF
541: PFSRMADLSQ TITVMPKAPN EAQMETLLNT VGFIGAQTGF PAINLTVTDD GSTIQGKDAD
601: IMIIGGIPDK LKDDKQIDLL VQATESWVKT PMRQTPFPGI VPDESDRAAE TRSTLTSSGA
661: MAAVIGFQSP YNDQRSVIAL LADSPRGYEM LNDAVNDSGK RATMFGSVAV IRESGINSLR
721: VGDVYYVGHL PWFERVWYAL ANHPILLAVL AAISVILLAW VLWRLLRIIS RRRLNPDNE