4.D.3.1.6
Core cellulose synthase complex, BcsA/BcsB (Morgan et al. 2013; Omadjela et al. 2013). BcsA (YhjO) is an 10 - 12 TMS protein with 4 N-terminal TMSs, the glycosyl transferase domain and 4 C-terminal TMSs. It is believed to both synthesize the glycosyl linkages and transport the polysaccharide across the membrane (Römling and Galperin 2015). BcsB (YhjN) is a cellulose synthase regulator, a cyclic diGMP binding protein, with two TMSs at the N- and C-termini and the bulk of the protein in the periplasm. BcsC (TC# 1.B.55.3.1) is the outer membrane porin for the export of cellulose with an N-terminal α-TMS, a large N-terminal hydrophylic domain, and a C-terminal β-barrel domain. Two other proteins, an endoglucanase, BscZ or YhjM, and a diguanylate cyclase, YedQ (TC# 9.B.34.1.4) may play roles in cellulose biosynthesis (Imai et al. 2014). Cellulose is a component of the bacterial extracellular matrix (Zogaj et al. 2001) and plays roles in biofilm formation and cell adhesion (Hu et al. 2015).
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| Accession Number: | P37653 |
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| Protein Name: | Cellulose synthase catalytic subunit [UDP-forming] |
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| Length: | 872 |
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| Molecular Weight: | 99785.00 |
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| Species: | Escherichia coli (strain K12) [83333] |
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| Number of TMSs: | 8 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
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| Substrate |
(1->4)-beta-D-glucan |
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1: MSILTRWLLI PPVNARLIGR YRDYRRHGAS AFSATLGCFW MILAWIFIPL EHPRWQRIRA
61: EHKNLYPHIN ASRPRPLDPV RYLIQTCWLL IGASRKETPK PRRRAFSGLQ NIRGRYHQWM
121: NELPERVSHK TQHLDEKKEL GHLSAGARRL ILGIIVTFSL ILALICVTQP FNPLAQFIFL
181: MLLWGVALIV RRMPGRFSAL MLIVLSLTVS CRYIWWRYTS TLNWDDPVSL VCGLILLFAE
241: TYAWIVLVLG YFQVVWPLNR QPVPLPKDMS LWPSVDIFVP TYNEDLNVVK NTIYASLGID
301: WPKDKLNIWI LDDGGREEFR QFAQNVGVKY IARTTHEHAK AGNINNALKY AKGEFVSIFD
361: CDHVPTRSFL QMTMGWFLKE KQLAMMQTPH HFFSPDPFER NLGRFRKTPN EGTLFYGLVQ
421: DGNDMWDATF FCGSCAVIRR KPLDEIGGIA VETVTEDAHT SLRLHRRGYT SAYMRIPQAA
481: GLATESLSAH IGQRIRWARG MVQIFRLDNP LTGKGLKFAQ RLCYVNAMFH FLSGIPRLIF
541: LTAPLAFLLL HAYIIYAPAL MIALFVLPHM IHASLTNSKI QGKYRHSFWS EIYETVLAWY
601: IAPPTLVALI NPHKGKFNVT AKGGLVEEEY VDWVISRPYI FLVLLNLVGV AVGIWRYFYG
661: PPTEMLTVVV SMVWVFYNLI VLGGAVAVSV ESKQVRRSHR VEMTMPAAIA REDGHLFSCT
721: VQDFSDGGLG IKINGQAQIL EGQKVNLLLK RGQQEYVFPT QVARVMGNEV GLKLMPLTTQ
781: QHIDFVQCTF ARADTWALWQ DSYPEDKPLE SLLDILKLGF RGYRHLAEFA PSSVKGIFRV
841: LTSLVSWVVS FIPRRPERSE TAQPSDQALA QQ