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3.A.1.12.3
Choline porter

Accession Number:P39775
Protein Name:OPBD aka OpuBD aka PROZ
Length:226
Molecular Weight:23928.00
Species:Bacillus subtilis [1423]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate choline

Cross database links:

RefSeq: NP_391250.1   
Entrez Gene ID: 936226   
Pfam: PF00528   
KEGG: bsu:BSU33700   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (4)

[1] “Characterization of a chimeric proU operon in a subtilin-producing mutant of Bacillus subtilis 168.”  Lin Y.et.al.   7592481
[2] “Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.”  Kappes R.M.et.al.   10216873
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “Determination of the sequence of spaE and identification of a promoter in the subtilin (spa) operon in Bacillus subtilis.”  Chung Y.J.et.al.   1400221

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MNVLEQLMTY YAQNGSYVMD EFGRHFLMSA YGVLFAAVVG VPAGILIAHF RRLSAWVFAV 
61:	TNVIQTIPAL AMLAVLMLVM GLGANTVILS LFLYSLLPII RNTYTGIISI EHAYLESGKA 
121:	MGMTKFQVLR MVELPLALSV IMAGLRTALV IAIGITAIGT FVGAGGLGDM IVRGSNATNG 
181:	TAIILAGAIP TAVMAVGADL LMAWLERALS PVKKKRTGAK HVQSAA