TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.C.1.2.1
The TolA energy-transducing system. The H+-channel-forming TolQ/R proteins are listed under TC# 1.A.30.2.2.  Proteins of the complex listed here are TolA, TolB, YbgF and Pal.  They play a role in outer membrane stabilization and uptake of colicins, but not energizaton of outer membrane receptors (OMRs: TC# 1.B.14).  May function in phospholipid transport (SS Chng, personal communication).  Cells lacking the Tol-Pal complex exhibit defects in lipid asymmetry and accumulate excess phospholipids (PLs) in the OM. This imbalance in OM lipids is due to defective retrograde PL transport in the absence of a functional Tol-Pal complex. Thus, cells ensure the assembly of a stable OM by maintaining an excess flux of PLs to the OM only to return the surplus to the inner membrane via transport mediated by the Tol-Pal complex (Shrivastava et al. 2017).  The specific function of the Tol-Pal complex may be to transport lipids from the outer leaflet of the outer membrane to the inner leaflet of the outer member. The Tol-Pal complex is essential to maintain polar localization of the MCP chemotaxis receptor complex (Santos et al. 2014). The Tol-Pal complex, energized by TolQRA, and using the outer membrane proteins, BtuB and OmpF as receptors, is responsible for the uptake of colicin ColE9 and other bacteriocins; in this process, the complex in the outer membrane bridges and immobilizes the complex components in the inner membrane (Rassam et al. 2018).

Accession Number:P45955
Protein Name:YbgF
Length:263
Molecular Weight:28231.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate phospholipid

Cross database links:

DIP: DIP-11392N
RefSeq: AP_001380.1    NP_415270.1   
Entrez Gene ID: 947227   
BioCyc: EcoCyc:EG12854-MONOMER    ECOL168927:B0742-MONOMER   
KEGG: ecj:JW0732    eco:b0742   

Gene Ontology

GO:0005488 F:binding

References (6)

[1] “The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL).”  Lazzaroni J.-C.et.al.   1574003
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Detection of new genes in a bacterial genome using Markov models for three gene classes.”  Borodovsky M.et.al.   7567469
[6] “Defining the role of the Escherichia coli chaperone SecB using comparative proteomics.”  Baars L.et.al.   16352602
Structure:
2WZ7   2XDJ   6G5S     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MSSNFRHQLL SLSLLVGIAA PWAAFAQAPI SSVGSGSVED RVTQLERISN AHSQLLTQLQ 
61:	QQLSDNQSDI DSLRGQIQEN QYQLNQVVER QKQILLQIDS LSSGGAAAQS TSGDQSGAAA 
121:	STTPTADAGT ANAGAPVKSG NANTDYNAAI ALVQDKSRQD DAMVAFQNFI KNYPDSTYLP 
181:	NANYWLGQLN YNKGKKDDAA YYFASVVKNY PKSPKAADAM FKVGVIMQDK GDTAKAKAVY 
241:	QQVISKYPGT DGAKQAQKRL NAM