2.C.1.2.1 The TolA energy-transducing system. The H+-channel-forming
TolQ/R proteins are listed under TC# 1.A.30.2.2. Proteins of the
complex listed here are TolA, TolB, YbgF and Pal. They play a role in
outer membrane stabilization and uptake of colicins, but not energizaton
of outer membrane receptors (OMRs: TC# 1.B.14). May function in
phospholipid transport (SS Chng, personal communication). Cells lacking
the Tol-Pal complex exhibit defects in lipid asymmetry and
accumulate excess phospholipids (PLs) in the OM. This imbalance in OM
lipids is due to defective retrograde PL transport in the absence of a
functional Tol-Pal complex. Thus, cells ensure the assembly of a stable
OM by maintaining an excess flux of PLs to the OM only to return the
surplus to the inner membrane via transport mediated by the Tol-Pal
complex (Shrivastava et al. 2017).
The specific function of the Tol-Pal complex may be to transport lipids
from the outer leaflet of the outer membrane to the inner leaflet of
the outer member. The Tol-Pal complex is essential to maintain polar
localization of the MCP chemotaxis receptor complex (Santos et al. 2014). The Tol-Pal complex, energized by TolQRA, and using the outer membrane proteins, BtuB and OmpF as receptors, is responsible for the uptake of colicin ColE9 and other bacteriocins; in this process, the complex in the outer membrane bridges and immobilizes the complex components in the inner membrane (Rassam et al. 2018).
|
Accession Number: | P45955 |
Protein Name: | YbgF |
Length: | 263 |
Molecular Weight: | 28231.00 |
Species: | Escherichia coli [83333] |
Number of TMSs: | 1 |
Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
Substrate |
phospholipid |
---|
DIP: |
DIP-11392N
|
RefSeq: |
AP_001380.1
NP_415270.1
|
Entrez Gene ID: |
947227
|
BioCyc: |
EcoCyc:EG12854-MONOMER
ECOL168927:B0742-MONOMER
|
KEGG: |
ecj:JW0732
eco:b0742
|
|
[1] “The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL).” Lazzaroni J.-C. et.al. 1574003
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.” Oshima T. et.al. 8905232
[3] “The complete genome sequence of Escherichia coli K-12.” Blattner F.R. et.al. 9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.” Hayashi K. et.al. 16738553
[5] “Detection of new genes in a bacterial genome using Markov models for three gene classes.” Borodovsky M. et.al. 7567469
[6] “Defining the role of the Escherichia coli chaperone SecB using comparative proteomics.” Baars L. et.al. 16352602
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1: MSSNFRHQLL SLSLLVGIAA PWAAFAQAPI SSVGSGSVED RVTQLERISN AHSQLLTQLQ
61: QQLSDNQSDI DSLRGQIQEN QYQLNQVVER QKQILLQIDS LSSGGAAAQS TSGDQSGAAA
121: STTPTADAGT ANAGAPVKSG NANTDYNAAI ALVQDKSRQD DAMVAFQNFI KNYPDSTYLP
181: NANYWLGQLN YNKGKKDDAA YYFASVVKNY PKSPKAADAM FKVGVIMQDK GDTAKAKAVY
241: QQVISKYPGT DGAKQAQKRL NAM