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2.A.108.1.5
High-affinity oxidase-dependent plasma membrane Fe2+-Fe3+ uptake transporter, Fio1/Fip1 (Frp1) (Singh et al., 2006). Metalloreductase responsible for reducing extracellular iron and copper prior to import. It catalyzes the reductive uptake of Fe3+-salts and Fe3+ bound to catecholate or hydroxamate siderophores. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane. Fio1 is a multicopper oxidase that contains three cupredoxin domains with eleven candidate iron-binding ligands, whereas Frp1 harbors a ferric reductase domain with three-candidate heme-binding ligands. The complex also participates in Cu2+ reduction and Cu+ uptake (Ahmad et al. 2022).

Accession Number:Q09920
Protein Name:Fio1
Length:622
Molecular Weight:69908.00
Species:Schizosaccharomyces pombe (Fission yeast) [4896]
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass type I membrane protein2 / Extracellular side3
Substrate iron(2+), iron(3+)

Cross database links:

RefSeq: NP_594494.1   
Entrez Gene ID: 2541558   
Pfam: PF00394    PF07731    PF07732   
KEGG: spo:SPAC1F7.08   

Gene Ontology

GO:0005783 C:endoplasmic reticulum
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005507 F:copper ion binding
GO:0016491 F:oxidoreductase activity
GO:0005515 F:protein binding
GO:0070627 P:ferrous iron import
GO:0033215 P:iron assimilation by reduction and transport
GO:0055114 P:oxidation reduction

References (2)

[1] “The genome sequence of Schizosaccharomyces pombe.”  Wood V.et.al.   11859360
[2] “An oxidase-permease-based iron transport system in Schizosaccharomyces pombe and its expression in Saccharomyces cerevisiae.”  Askwith C.et.al.   8995275

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FASTA formatted sequence 
1:	MNKFFSFPIL GLLLTCVRFV VAKERLFEWN VTDVYDVDPD GSGNSRWVIG VNNKWPIDPL 
61:	VVDYGDQVII KMTNSLANNR TTSLHSHGLF QKFTPYMDGV PQSTQCEIPP GATFYYNYTA 
121:	LQNGTYWVHS HDMSQYPDGL RTPFIINALE EPYDYDEEYI ISMTDWYYTP FNILVPDEFK 
181:	TWKNPTGAEP VPDTGLFNDT ANATFAMEPG KTYRLRFINI GAFNNYDVMI EDHNMTIIEV 
241:	DGEYTEPQEV SSIHLTVAQR YSVLVTAKNS TDRNYAITAY MDESLFDTIP DNYNPNVTAW 
301:	LSYNSDASYD LGPDIDEIDS YDDAELNPLY SWDVTESNHS INIWFDFFTL GDGANYAEIN 
361:	DSSYVFPKVP SIMIANSTNV DGYNLEPVTY GPYTNAYIFE YGDVVDVIID NHDTGKHPFH 
421:	LHGHTFQVLE RGEENAGLYS DQESHTYYDN PMRRDTVEIE PGSFIVIRFI ADNPGAWVIH 
481:	CHIEWHMESG LLATFIEAPE MIPSISSPDF VKEQCMLDGV PTIGNGAGNY KNISDLSGAP 
541:	SPPGEMPAGW TSKAIGTMAA CVISACIGMG SIIFYGASIH PVPTEELDEN DDLQEAALEN 
601:	AAMFLDTDKA VEKVVEGKDE IK