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3.A.21.1.1
The C-terminal tail-anchored (TA) membrane protein biogenesis/insertion complex, Get1/Get2/Get3 (Stefer et al., 2011; Kubota et al. 2012; Wang et al. 2014). The ATPase (Get3) is homologous to ArsA of the arsenite exporters (Castillo and Saier, 2010). Get1 and Get2 but not Get3 are required for mitochondrial autophagy, either because of a requirement for Get1/2-dependent TA protein(s), or because the Get1/2 complex itself acts specifically in mitophagy (Onishi et al. 2018). Get3 serves as a chaparone protein, feeding into Get1/Get2 (McDowell et al. 2020). There appear to be distinctive pathways of mammalian mitophagy (Xu et al. 2020). The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER (Heo et al. 2023).  Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defects (Oleinik et al. 2023).

Accession Number:Q12154
Protein Name:GET3 aka ARR4 aka YDL100C aka D2371
Length:354
Molecular Weight:39354.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate protein polypeptide chain

Cross database links:

DIP: DIP-3908N DIP-3908N
RefSeq: NP_010183.1   
Entrez Gene ID: 851458   
KEGG: sce:YDL100C    sce:YDL100C   

Gene Ontology

GO:0043529 C:GET complex
GO:0005794 C:Golgi apparatus
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0005085 F:guanyl-nucleotide exchange factor activity
GO:0046872 F:metal ion binding
GO:0005515 F:protein binding
GO:0006875 P:cellular metal ion homeostasis
GO:0000750 P:pheromone-dependent signal transduction inv...
GO:0045048 P:protein insertion into ER membrane
GO:0046685 P:response to arsenic
GO:0009408 P:response to heat
GO:0010038 P:response to metal ion
GO:0006890 P:retrograde vesicle-mediated transport, Golg...
GO:0015446 F:arsenite-transporting ATPase activity
GO:0000750 P:pheromone-dependent signal transduction involved in conjugation with cellular fusion
GO:0006620 P:posttranslational protein targeting to membrane
GO:0046685 P:response to arsenic-containing substance
GO:0006890 P:retrograde vesicle-mediated transport, Golgi to ER

References (26)

[1] “The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames.”  Boskovic J.et.al.   8923743
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[3] “Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.”  Hu Y.et.al.   17322287
[4] “Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.”  Ho Y.et.al.   11805837
[5] “The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance.”  Shen J.et.al.   12680698
[6] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[7] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[8] “Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.”  Schuldiner M.et.al.   16269340
[9] “The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.”  Auld K.L.et.al.   16816426
[10] “The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol.”  Metz J.et.al.   16260785
[11] “The GET complex mediates insertion of tail-anchored proteins into the ER membrane.”  Schuldiner M.et.al.   18724936
[12] “The structural basis of tail-anchored membrane protein recognition by Get3.”  Mateja A.et.al.   19675567
[13] “Model for eukaryotic tail-anchored protein binding based on the structure of Get3.”  Suloway C.J.M.et.al.   19706470
[14] “The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames.”  Boskovic J.et.al.   8923743
[15] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[16] “Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.”  Hu Y.et.al.   17322287
[17] “Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.”  Ho Y.et.al.   11805837
[18] “The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance.”  Shen J.et.al.   12680698
[19] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[20] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[21] “Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.”  Schuldiner M.et.al.   16269340
[22] “The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.”  Auld K.L.et.al.   16816426
[23] “The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol.”  Metz J.et.al.   16260785
[24] “The GET complex mediates insertion of tail-anchored proteins into the ER membrane.”  Schuldiner M.et.al.   18724936
[25] “The structural basis of tail-anchored membrane protein recognition by Get3.”  Mateja A.et.al.   19675567
[26] “Model for eukaryotic tail-anchored protein binding based on the structure of Get3.”  Suloway C.J.M.et.al.   19706470
Structure:
2WOJ   3A36   3A37   3H84   3IDQ   3B2E   3SJA   3SJB   3SJC   3SJD   [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MDLTVEPNLH SLITSTTHKW IFVGGKGGVG KTTSSCSIAI QMALSQPNKQ FLLISTDPAH 
61:	NLSDAFGEKF GKDARKVTGM NNLSCMEIDP SAALKDMNDM AVSRANNNGS DGQGDDLGSL 
121:	LQGGALADLT GSIPGIDEAL SFMEVMKHIK RQEQGEGETF DTVIFDTAPT GHTLRFLQLP 
181:	NTLSKLLEKF GEITNKLGPM LNSFMGAGNV DISGKLNELK ANVETIRQQF TDPDLTTFVC 
241:	VCISEFLSLY ETERLIQELI SYDMDVNSII VNQLLFAEND QEHNCKRCQA RWKMQKKYLD 
301:	QIDELYEDFH VVKMPLCAGE IRGLNNLTKF SQFLNKEYNP ITDGKVIYEL EDKE