2.A.6.2.40
MDR pump, AdeABC (Acinetobacter drug efflux B = AdeB).It exports chloramphenicol and tetracycline (Hassan et al., 2011), but also confers resistance to meropenem, tigecycline and ceftazidime (Peleg et al. 2007; Provasi Cardoso et al. 2016). Morgan et al. 2021 reported six structures of the trimeric AdeB multidrug efflux pump in the presence of ethidium bromide using single-particle cryo-EM. These structures allowed them to directly observe various novel conformational states of the AdeB trimer. The transmembrane region of trimeric AdeB can associate with formation of a trimeric assembly or dissociated into "dimer plus monomer" and "monomer plus monomer plus monomer" configurations. A single AdeB protomer can simultaneously anchor a number of ethidium ligands, and different AdeB protomers can bind ethidium molecules simultaneously. A drug transport mechanism was proposed that involves multiple multidrug-binding sites and various transient states of the AdeB membrane protein. This suggests that each AdeB protomer within the trimer binds and exports drugs independently (Morgan et al. 2021).
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| Accession Number: | Q2FD70 |
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| Protein Name: | AdeB, RND |
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| Length: | 1036 |
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| Molecular Weight: | 112624.00 |
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| Species: | Acinetobacter baumannii [470] |
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| Number of TMSs: | 12 |
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| Substrate |
chloramphenicol, tetracycline, tigecycline, ceftazidime, meropenem, ethidium |
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1: MMSQFFIRRP VFAWVIAIFI IIFGLLSIPK LPIARFPSVA PPQVNISATY PGATAKTIND
61: SVVTLIEREL SGVKNLLYYS ATTDTSGTAE ITATFKPGTD VEMAQVDVQN KIKAVEARLP
121: QVVRQQGLQV EASSSGFLML VGINSPNNQY SEVDLSDYLV RNVVEELKRV EGVGKVQSFG
181: AEKAMRIWVD PNKLVSYGLS ISDVNNAIRE NNVEIAPGRL GDLPAEKGQL ITIPLSAQGQ
241: LSSLEQFKNI SLKSKTNGSV IKLSDVANVE IGSQAYNFAI LENGKPATAA AIQLSPGANA
301: VKTAEGVRAK IEELKLNLPE GMEFSIPYDT APFVKISIEK VIHTLLEAMV LVFIVMYLFL
361: HNVRYTLIPA IVAPIALLGT FTVMLLAGFS INVLTMFGMV LAIGIIVDDA IVVVENVERI
421: MATEGLSPKD ATSKAMKEIT SPIIGITLVL AAVFLPMAFA SGSVGVIYKQ FTLTMSVSIL
481: FSALLALILT PALCATILKP IDGHHQKKGF FAWFDRSFDK VTKKYELMLL KIIKHTVPMM
541: VIFLVITGIT FAGMKYWPTA FMPEEDQGWF MTSFQLPSDA TAERTRNVVN QFENNLKDNP
601: DVKSNTAILG WGFSGAGQNV AVAFTTLKDF KERTSSASKM TSDVNSSMAN STEGETMAVL
661: PPAIDELGTF SGFSLRLQDR ANLGMPALLA AQDELMAMAA KNKKFYMVWN EGLPQGDNIS
721: LKIDREKLSA LGVKFSDVSD IISTSMGSMY INDFPNQGRM QQVIVQVEAK SRMQLKDILN
781: LKVMGSSGQL VSLSEVVTPQ WNKAPQQYNR YNGRPSLSIA GIPNFDTSSG EAMREMEQLI
841: AKLPKGIGYE WTGISLQEKQ SESQMAFLLG LSMLVVFLVL AALYESWAIP LSVMLVVPLG
901: IFGAIIAIMS RGLMNDVFFK IGLITIIGLS AKNAILIVEF AKMLKEEGMS LIEATVAAAK
961: LRLRPILMTS LAFTCGVIPL VIATGASSET QHALGTGVFG GMISATILAI FFVPVFFIFI
1021: LGAVEKLFSS KKKISS