1.I.1.1.5
The nuclear envelope consists of the outer and the inner nuclear membrane, the nuclear lamina and the nuclear pore complexes, which regulate nuclear import and export (Batsios et al. 2019). The major constituent of the nuclear lamina of Dictyostelium is the lamin NE81. It can form filaments like B-type lamins, and it interacts with Sun1, as well as with the LEM/HeH-family protein Src1. Sun1 and Src1 are nuclear envelope transmembrane proteins involved in the centrosome-nucleus connection and nuclear envelope stability at the nucleolar regions, respectively. In conjunction with a KASH-domain protein, Sun1 usually forms a so-called LINC complex. Two proteins with functions reminiscent of KASH-domain proteins at the outer nuclear membrane of Dictyostelium are known; interaptin which serves as an actin connector and the kinesin Kif9 which plays a role in the microtubule-centrosome connector, both of which lack the conserved KASH-domain. The link of the centrosome to the nuclear envelope is essential for the insertion of the centrosome into the nuclear envelope and appropriate spindle formation. Centrosome insertion is involved in permeabilization of the mitotic nucleus, which ensures access of tubulin dimers and spindle assembly factors (Batsios et al. 2019).
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| Accession Number: | Q555I8 |
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| Protein Name: | Kinesin-related protein 9 |
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| Length: | 1222 |
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| Molecular Weight: | 136066.00 |
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| Species: | Dictyostelium discoideum (Slime mold) [44689] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Membrane1 / Single-pass membrane protein2 |
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| Substrate |
protein, (ribonucleotide)n+m |
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1: MDNNNNNFST PKQPTINSTT GGQLRSRSNS SPSTSSISTP RNGSTTATTS SITNSIGKGS
61: LVFSTMENAK KTLNMDSLST PMSQSSKKRL SMNSSLLPPS SIPLPPSQQL SAKKDPSKRH
121: SSFISTTTTT SLGSRPSTPQ PNHTTNNNNN NNNGSNGNNV TSTNISSMLE SNNSEILSQN
181: QKVTLSTSSN TAFSSLPSST NNGNNPLSNS GGGNGNHHLV NSNSSTSTPS PTMFISTTSP
241: PPELSLMEGF NQHNLTTTTT TTTTTTNNTL NTSNGINSNS LSPSSHLNNG SNNNNNNNHL
301: NLNNRSSSPS PTPSSSSISG RRTPIQNFNS VGGVNITSKT QPLFSDREES IQAVCRFRPE
361: TAAEQTLGPS EKQLTIGTDQ TTIHITPTSS SSSSMAQAFR FSKVYQPNTT QESFYNEVGK
421: PLIDNIVNGY NVGIIAYGQT GAGKTFSLGF SGGEGNDSYN SYCNRSEFYN NSHYLYGSQQ
481: QQQQQQLLPS SWGIMPRIIK DLFIKQDEQQ SMNTPQRIKF TTKISYLEVY KEKVYDLLSE
541: GGVNDIEIRM ADGGFIVPDA VQSSIQTFTD FLTHLQHIEK NRKIAETKKN MASSRSHAIF
601: IVSLLKEDLE NKMTVTSLLY LVDLAGSESA SKIDGTTSKI EETKSINKSL YALGGVIEDM
661: SKNSKHVRYR DSKLTQLLQQ CFGGNSKTCL IVNCSSSNHE SVIRETIQSL NFGQRAQSVK
721: NKPLQNVEES HSELKAKLRE LNKDIETYKK FIEKISINSG VIPPSSVATY ITQLQNACEE
781: LKKKNDRLQE DIINLEEENS DLPKKFNVLN KNNVSQIEIL TQELELSKQI NQEQLLKIEQ
841: YSQKYQATNE EIQKSFNHRQ QLVNDLHDSN EKSKQLELKL KDALLNSKSE SDEIRLKLTK
901: ALEESTDKDQ RINTLESNKQ KWKSKCNEVV RQSKELQDRL NILQSTYSNS VDSLSNSSLN
961: NNNNESLEEI KKLKLNISSL TNQHIEREKS LQKEIDLARS KHTEQTLILK NLKTQIDTLT
1021: NKLEIQFPTI VNNNQQQQQS TQSSSSSSIE SNLIISMNTI EVLFEKICKN QEQTNTIIST
1081: NVESIMGKLV EIDENEEFIR KEKDRERERA ENLKNTLEDA QSNFLKQQEE IKQFIETQLK
1141: NKNNINNNNN IKNNNNNNKL KSKKVGSSSS SSSNIGSSIC INILFFLIIL VILFFLMVAV
1201: GLTIQNQDYN SQYRSSYFFK TT